Vectors, Plasmids and Libraries
在分子複製中,載體是用來將外源遺傳物質人工輸送到另一個細胞中的 DNA 分子。四種主要的載體類型為質粒、病毒載體、宇宙粒(cosmid)以及人工染色體,其中最常使用的是質粒。載體可用於複製,一些載體專門為此設計,另一些則專為轉錄和蛋白質表達而製作。較簡單的轉錄載體則用於擴增其插入的遺傳物質。
Expression vectors, pGEX
Supplier: GE HEALTHCARE
The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.
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E. coli BL21
Supplier: GE HEALTHCARE
The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.
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M13KO7 Helper Phage, Cytiva
Supplier: GE HEALTHCARE
M13 phage for the GST expression system.
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Polyadenylic acid (poly-A)
Supplier: GE HEALTHCARE
Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.



