"PROMEGA"

Supplier: PROMEGA

The Griess Reagent System measures nitrite (NO²⁻), which is one of two primary stable and non volatile breakdown products of nitric oxide (NO).

Supplier: PROMEGA

Antibiotic G-418 sulfate is an aminoglycosidic antibiotic toxic to both prokaryotic and eukaryotic cells.

Supplier: PROMEGA

The ROS-Glo™ H₂O₂ Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H₂O₂), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions. A derivatised luciferin substrate is incubated with sample and reacts directly with H₂O₂ to generate a luciferin precursor. The ROS-Glo™ detection solution converts the precursor to luciferin and provides Ultra-Glo™ Recombinant Luciferase to produce light signal that is proportional to the level of H₂O₂ present in the sample.

Supplier: PROMEGA

Endoproteinase hydrolyses peptide bonds on the N-terminal side of aspartic and cysteic acid residues (Asp and Cys).

Supplier: PROMEGA

The Bio-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous reagent for the detection of firefly luciferase reporter gene expression in the ADCC Reporter Bioassay.

Supplier: PROMEGA

Polyclonal secondary antibody raised in goat, immunoaffinity-purified using immobilized antigens and conjugated to alkaline phosphatase (AP) enzyme.

Supplier: PROMEGA

Immobilised trypsin provides a fast and convenient method for digesting a range of concentrations of purified protein or complex protein mixtures. Digested peptides are easily separated from the immobilised trypsin as they flow through the spin column into the collection tube. Immobilised trypsin is easily removed from the peptide solution because the trypsin does not pass though the column frit.

Supplier: PROMEGA

Trypsin Gold, mass spectrometry grade, has been manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion. The specificity of the purified trypsin is further improved by TPCK treatment, which inactivates chymotrypsin. The treated trypsin is then purified by affinity chromatography and lyophilised.

Supplier: PROMEGA

ViaFect™ transfection reagent is designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. ViaFect™ Transfection Reagent has been shown to transfect cell lines that are traditionally thought of as difficult to transfect (such as hematopoietic, primary and iPSC stem cell lines) with relatively high efficiency. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex.

Supplier: PROMEGA

GoTaq® DNA polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and, in some cases superior to that of standard Taq polymerase.

Supplier: PROMEGA

The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 to 20 moles of luciferase can be measured under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) assay.

Supplier: PROMEGA

The MagneSphere® Technology magnetic separation stands can be used in conjunction with any of the PolyATtract® systems and are ideal for applications requiring multiple paramagnetic isolations of biomolecules.

Supplier: PROMEGA

The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells. A change in GSH levels is important in the assessment of toxicological responses and is an indicator of oxidative stress, potentially leading to apoptosis or cell death. The assay provides a simple, rapid multiwell-plate format where stable luminescent signals are correlated with either the total GSH or the GSSG concentration of a sample directly in culture wells.

Supplier: PROMEGA

dNTP Mix is a solution containing sodium salts of dATP, dCTP, dGTP and dTTP, each at 10 mM in water at pH 7,5. The total concentration of nucleotides is 40 mM. One microliter of the dNTP Mix in a 50 μl reaction will give a final dNTP concentration of 200 μM for each dNTP.

Supplier: PROMEGA

Nuclease-Free Water is an essential component of molecular biology experiments.

Supplier: PROMEGA

Wheat germ extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors).