"PROMEGA"

Supplier: PROMEGA

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer)

Supplier: PROMEGA

IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). IdeZ Protease is an immunoglobulin-degrading enzyme from Streptococcus equi subspecies zooepidemicus.

Supplier: PROMEGA

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System is designed for this purpose of providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media, and does not require sample preprocessing. Throughput rates of several thousand samples per hour may be achieved with high reproducibility under standard laboratory conditions.

Supplier: PROMEGA

CellTiter 96® AQueous MTS Reagent Powder is a novel tetrazolium compound used in colorimetric assays to determine the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.

Supplier: PROMEGA

T4 RNA ligase catalyses the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43,5 kDa. It also catalyses the addition of [5'-³²P] nucleoside 3',5'-bis (phosphate) onto single-stranded RNA.

Supplier: PROMEGA

Western Blue® stabilised substrate for alkaline phosphatase is a stable, ready to use substrate for Western blots and immunoscreening.

Supplier: PROMEGA

The CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.

Supplier: PROMEGA

The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantisation of a stable luminescent signal from two reporter genes in a single sample. The system is optimised for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media.

Supplier: PROMEGA

Human Genomic DNA is isolated from multiple anonymous donors.

Supplier: PROMEGA

Mouse genomic DNA is isolated from whole blood from disease-free mice.

Supplier: PROMEGA

This EDTA solution is a chelator of divalent cations and is suitable for biochemistry and molecular biology applications

Supplier: PROMEGA

The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample.

Supplier: PROMEGA

The Caspase-Glo® 3/7 assay is a homogeneous luminescent assay that measures caspase-3/7 activities. The assay provides a proluminescent caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a reagent optimised for caspase-3/7 activity, luciferase activity and cell lysis. This liberates free aminoluciferin, which is consumed by the luciferase, generating a 'glow-type' luminescent signal. The signal is proportional to caspase-3/7 activity. It is designed for use with multiwell plate formats using either purified enzyme or cells in culture.

Supplier: PROMEGA

The Griess Reagent System measures nitrite (NO²⁻), which is one of two primary stable and non volatile breakdown products of nitric oxide (NO).

Supplier: PROMEGA

Antibiotic G-418 sulfate is an aminoglycosidic antibiotic toxic to both prokaryotic and eukaryotic cells.

Supplier: PROMEGA

The ROS-Glo™ H₂O₂ Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H₂O₂), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions. A derivatised luciferin substrate is incubated with sample and reacts directly with H₂O₂ to generate a luciferin precursor. The ROS-Glo™ detection solution converts the precursor to luciferin and provides Ultra-Glo™ Recombinant Luciferase to produce light signal that is proportional to the level of H₂O₂ present in the sample.