"Buffer Solution - Red"

Supplier: VWR Chemicals

Phenol red : ammonium sulphate (0.002 : 0.005) % in aqueous solution Reag. Ph. Eur. 1063603, buffered pH 4.7

Supplier: VWR Chemicals

Phenol red : ammonium sulphate (0.002 : 0.01) % in aqueous solution Reag. Ph. Eur. 1063604, buffered pH 4.7

Supplier: VWR Chemicals

Solutions prepared from AnalaR® NORMAPUR® grade analytical reagents.

Supplier: Avantor

The quality of many BAKER ANALYZED™ reagents meets or exceeds the requirements set forth by the American Chemical Society (ACS).

Supplier: HACH

For calibration of pH meters

Supplier: MERCK MILLIPORE

Materials for the precise calibration and monitoring of pH meters.

Supplier: HAMILTON BONADUZ

For full information concerning health and safety data please contact Avantor or Hamilton directly.

Supplier: CYTIVA HYCLONE

Balanced salt solutions for cell culture applications.

Supplier: HACH

The unit-dose powder pillows assure buffer freshness and eliminate the possibility of contamination. Each powder pillow prepares 50 ml of buffer solutions of respective pH.

Supplier: BDH US PRODUCED

Buffers are available as colourless and coloured solutions.

Supplier: MACRON AVANTOR BRAND

BuffAR® StandARd® pH reference solution, pH value: 4,01, ±0,01, Macron Fine Chemicals™ BuffAR®, Red, Plastic bottle

Supplier: CYTIVA HYCLONE

Balanced salt solution used for multiple cell culture applications, (liquid form).

Supplier: THERMO ORION

Perform accurate, reproducible pH calibrations with Orion™ pH buffers.

Supplier: EBIOSCIENCE

5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. APC-eFluor® emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

Supplier: CYTIVA HYCLONE

Balanced salt solution used for multiple cell culture applications.

Supplier: VWR Collection

VWR® Fluorometer for quantitation of DNA, RNA, and Proteins. Compact with touch screen control panel. Compatible with VWR® quantitation kits and kits from leading suppliers.