Glutathione Sepharose High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.
- 34 µm bead size for improved efficiency and resolution
- Fast, one-step purification
- Mild conditions during the purification process preserves protein antigenicity and function
GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. Fast, one-step purification of GST-tagged proteins and other glutathione S-trasferase or glutathione-dependent proteins.
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- pH range:3 - 12
- Matrix:Highly cross-linked agarose, 6%
- Ligand:Glutathione
- Storage temperature:4 - 30 °C
- Binding capacity:>Approx. 10 mg GST-tagged protein/ml
- Flow rate:<600 cm/h