HiTrap rProtein A FF columns are prepacked with rProtein A Sepharose Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.
- Quick, convenient preparative purification of monoclonal antibodies
- Well-established protein A-based resin used in many approved MAb processes
- Simple operations with a syringe, pump, or high-performance chromatography system, such as ÄKTA design
Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.
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- Catalog No:
- GEHE17-5079-01
- GEHE17-5079-02
- GEHE17-5080-01
- GEHE17-5080-02
- Max. pressure:
- 5 bar
- 5 bar
- 5 bar
- 5 bar
- pH range:
- 2 - 9
- 2 - 9
- 2 - 9
- 2 - 9
- Storage:
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol
- 4 to 30 °C, 20% Ethanol