The four large squares in the corners marked 'L' for counting leucocytes are each subdivided into 16 squares with 0,25 mm sides.

• The ruling shows nine large squares of one mm² each
• The large square in the center is subdivided into 16 squares with 0,25 mm sides
• Each group square consists of 16 mini squares with 0,05 mm sides, each having an area of 0,0025 mm²

The five group squares in the large central square are used for counting thrombocytes and erythrocytes.

Contrary to the more advanced Improved Neubauer counting chambers, the counting area of each group square is limited by the outer one of the triple boundary lines.