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Specifications
- Pk:1 KIT
- Description:illustra™ TempliPhi™ DNA Sequencing Template Amplification Kit
- Storage conditions:Denature buffer and control DNA at -70°C or -15 to -30°C. TempliPhi premix at -70°C (do not store TempliPhi premix at -15 to -30°C). Thaw premix on ice, maintain at 0-4°C during handling (do not allow premix to warm above 4°C prior to amplification)
- No. of reactions:2000
Specifications
About this item
TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.
- Prepare circular DNA templates for cycle sequencing, cloning, and transformation in 4 to 6 hours
- Generate microgram quantities of template DNA from picogram amounts of starting material
- Use amplified DNA directly for cycle sequencing without purification
- Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA
- Simple protocol reduces time, labour, and consumables needed for template preparation, and workflow enables easy automation