IdeS Protease
IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). IdeZ Protease is an immunoglobulin-degrading enzyme from Streptococcus equi subspecies zooepidemicus.
- Antibody fragmentation and characterisation, simplified
- Highly specific IgG cleavage
- Rapid generation of highly pure fragments
- Frozen protease is supplied at a concentration of 50 U/µl
IdeS protease: IdeS protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). It is an engineered recombinant protease overexpressed in E. coli that cleaves Immunoglobulin G (IgG) with high specificity at a single site below the hinge region, yielding F(ab’)2 and Fc fragments. The protocol for a standard reaction is to add the IdeS protease to the IgG sample, add 1 unit of IdeS protease per 1µg of IgG to be digested and incubate the sample at 37 °C for 30 to 60 minutes in a neutral pH buffer. IdeZ Protease: IdeZ protease is an immunoglobulin-degrading enzyme from Streptococcus equi subspecies zooepidemicus. It is an engineered recombinant protease overexpressed in E. coli. Like IdeS Protease, IdeZ Protease specifically cleaves IgG molecules below the hinge region to yield F(ab′)2 and Fc fragments. However, IdeZ protease has significantly improved activity against mouse IgG2a and IgG3 subclasses compared to IdeS protease.
IdeZ protease is an engineered recombinant protease overexpressed in E. coli. Like IdeS protease, IdeZ protease specifically cleaves IgG molecules below the hinge region to yield F(ab′)2 and Fc fragments. However, IdeZ Protease has significantly improved activity against mouse IgG2a and IgG3 subclasses compared to IdeS Protease.
coli that cleaves Immunoglobulin G (IgG) with high specificity at a single site below the hinge region, yielding F(ab’)2 and Fc fragments. The protocol for a standard reaction is to add the IdeS Protease to the IgG sample, add one unit of IdeS Protease per 1 µg of IgG to be digested, and incubate the sample at 37 °C for 30 to 60 minutes in a neutral pH buffer.
- Pk:1
- Pk Info:5000 units
- Cat. No.:PROMV7511
- Storage conditions:Store IdeS protease at –30...–10 °C, store IdeZ protease at –30...–10 °C
- Enzyme name:IdeS protease
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