Some Products May Appear Restricted
To ensure a smooth and speedy checkout, please log in to your account. Some items may show as restricted simply because you're not logged in.
If you do not have an account, you can register using our registration webform (https://www.avantorsciences.com/us/en/login/register)
If you're still seeing restrictions after logging in, certain products—like chemicals or medical devices—require additional account verification steps to be able to place an order. Some items may additionally require a specific license or customer documentation; additional documentation will be requested for these items prior to shipment.
Specifications
- Particle Size:34 µm
- Capacity:100 µl
- Description:His SpinTrap™
- Seq:01
- Binding capacity:At least 40 mg histidine-tagged protein/ml medium
- Storage:4 to 30 °C, 0,15% Kathon CG
- Pk:50
- Sample volume:0,6 ml
Specifications
About this item
His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
- For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening
- Ni Sepharose High Performance in prepacked spin columns
- Yields up to 750 µg pure protein per column
- Allows direct purification of both clarified and unclarified cell lysates
Purification of unclarified samples on His SpinTrap columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.
His SpinTrap columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap together with buffers prepared using His Buffer Kit.
The columns are prepacked with Ni Sepharose High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose High Performance resin is negligible.