Griess reagent system
PROMG2930
: PROMEGA
:
- Pk:1.000 Assays
- Description:Griess Reagent System
The Griess Reagent System measures nitrite (NO²⁻), which is one of two primary stable and non volatile breakdown products of nitric oxide (NO).
- Assay uses diazotisation reaction
- Based on a chemical reaction that uses sulphanilamide and N-1-naphthylethylenediamine dihydrochloride (NED) under acidic conditions
- Nitrite sensitivity dependent on matrix
Nitric oxide is an important physiological messenger and effector molecule in many biological systems including immunological, neuronal, and cardiovascular tissues. This system detects NO²⁻ in a variety of biological and experimental liquid matrices such as plasma, serum, urine, and tissue culture medium.
: Includes 50 ml Sulphanilamide solution (2×25 ml), 50 ml NED solution (2×25 ml), and 1 ml Nitrite standard (0,1 M sodium nitrite).