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Specifications
- Pk:1.000 Assays
- Description:CytoTox 96® Non-Radioactive Cytotoxicity Assay
Specifications
About this item
The CytoTox 96® Non-Radioactive Cytotoxicity Assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [⁵¹Cr] is released in radioactive assays.
- Non-radioactive, time-saving assay
- Highly sensitive assay
- Low-level damage to cell membranes
Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay that results in the conversion of a tetrazolium salt (INT) into a red formazan product. The amount of colour formed is proportional to the number of lysed cells. Visible wavelength absorbance data are collected using a standard 96-well plate reader.
The CytoTox 96® Non-Radioactive Cytotoxicity Assay requires no radioactive waste disposal or [⁵¹Cr] and eliminates labeling of target cells prior to conducting experiments. Standard equipment can be used to collect absorbance (visible wavelength) data with a standard 96-well plate reader.
The assay can be used to measure membrane integrity for cell-mediated cytotoxicity assays in which a target cell is lysed by an effector cell, or to measure lysis of target cells by bacteria, viruses, proteins, chemicals, etc.