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Specifications
- Antibody type:Primary
- Antigen symbol:CD95 (APO-1/Fas)
- Clonality:Monoclonal
- Clone:DX2
- Conjugation:PE (Phycoerythrin)-Cy5®
- Flow cytometry:Yes
- Host:Mouse
- Isotype:IgG1 kappa
- Reactivity:Human,Dog
- Form:Liquid
- Gene ID:355
- Antigen synonyms:FASTM|APO-1|ALPS1A|TNFRSF6|CD95|Apo-1 Fas|APT1|FAS1
- Storage buffer:PBS, 0.2% BSA, 0.1% gelatin, 0.09% sodium azide, pH 7.2
- Storage temperature:4° C, store in dark, DO NOT FREEZE!
- Concentration:5 µl/Test
- Purification:Affinity chromatography
- Size:100 Tests
- Pk:100 Tests
Specifications
About this item
Description: The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. DX2 does not block binding of EOS9.1, another antibody specific for human CD95. The DX2 monoclonal is reported to recognize dog/canine CD95. Applications Reported: The DX2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This DX2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
Flow Cytometry: 5 µL (0.5 µg)/test
Type: Primary
Antigen: CD95 (APO-1/Fas)
Clonality: Monoclonal
Clone: DX2
Conjugation: PE (Phycoerythrin)-Cy5®
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human, Canine