The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 to 20 moles of luciferase can be measured under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) assay.

• Eight or more orders of magnitude of enzyme concentration
• Sensitive up to 10^-20 moles of luciferase
• Non-radioactive
• Perform cell lysis, sample preparation and assays in five minutes

The Luciferase Assay Reagent generates light that is nearly constant for at least one minute and so is compatible with measuring firefly luciferase in a single-tube or in a multiwell plate luminometer with an auto-injector.