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Specifications
- Antibody type:Primary
- Antigen name:CD27 molecule
- Antigen symbol:CD27
- Clonality:Monoclonal
- Clone:O323
- Flow cytometry:Yes
- Host:Mouse
- Isotype:IgG1 kappa
- Reactivity:Human
- Form:Liquid
- Gene ID:939
- Antigen synonyms:CD27 antigen|T-cell activation antigen CD27|tumor necrosis factor receptor superfamily member 7|CD27L receptor|T cell activation antigen S152|T cell activation antigen CD27
- Storage buffer:PBS, 0.2% BSA, 0.1% gelatin, 0.09% sodium azide, pH 7.2
- Storage temperature:4° C, store in dark, DO NOT FREEZE!
- Concentration:5 µl/Test
- Purification:Affinity chromatography
- Size:100 Tests
- Pk:100 Tests
Specifications
About this item
Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction. Applications Reported: This O323 antibody has been reported for use in flow cytometric analysis. Applications Tested: This O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser CD27 (50 kDa) is a member of the tumor necrosis factor (TNF) receptor superfamily. CD40 and CD30 are also members of the TNF receptor superfamily. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is a membranebound receptor, but a soluble form of CD27 is also produced.
Flow Cytometry: 5 µL (0.06 µg)/test
Type: Primary
Antigen: CD27
Clonality: Monoclonal
Clone: O323
Conjugation: PE (Phycoerythrin)rCP-eFluor 710
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human