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- Antibody type:Primary
- Antigen name:CD69 molecule
- Antigen symbol:CD69
- Clonality:Monoclonal
- Clone:FN50
- Flow cytometry:Yes
- Host:Mouse
- Isotype:IgG1 kappa
- Reactivity:Human
- Form:Liquid
- Gene ID:969
- Antigen synonyms:EA1|MLR-3|member C|AIM|GP32/28|CLEC2C|CD69 antigen|C-type lectin domain family 2|BL-AC/P26
- Storage buffer:PBS, 0.2% BSA, 0.1% gelatin, 0.09% sodium azide, pH 7.2
- Storage temperature:4° C, store in dark, DO NOT FREEZE!
- Concentration:5 µl/Test
- Purification:Affinity chromatography
- Size:100 Tests
- Pk:100 Tests
Description: The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets. Applications Reported: This FN50 antibody has been reported for use in flow cytometric analysis. Applications Tested: This FN50 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser Also called AIM or activation inducer molecule, CD69 is a gp28/34 disulfide bonded homodimer with a Mr of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride. The CD69 molecule is also present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen. It is involved in lymphocyte signal transduction.
Flow Cytometry: 5 µL (0.015 µg)/test
Type: Primary
Antigen: CD69
Clonality: Monoclonal
Clone: FN50
Conjugation: AP (Alkaline Phosphatase)C
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human