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Anti-CD69 Mouse Monoclonal Antibody (FITC (Fluorescein Isothiocyanate)) [clone: FN50]
Anti-CD69 Mouse Monoclonal Antibody (FITC (Fluorescein Isothiocyanate)) [clone: FN50]
Catalog # EBIO11-0699-42
Supplier:  EBIOSCIENCE
Anti-CD69 Mouse Monoclonal Antibody (FITC (Fluorescein Isothiocyanate)) [clone: FN50]
Catalog # EBIO11-0699-42
Supplier:  EBIOSCIENCE

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Specifications

  • Antibody type:
    Primary
  • Antigen name:
    CD69 molecule
  • Antigen symbol:
    CD69
  • Clonality:
    Monoclonal
  • Clone:
    FN50
  • Conjugation:
    FITC (Fluorescein Isothiocyanate)
  • Flow cytometry:
    Yes
  • Host:
    Mouse
  • Isotype:
    IgG1 kappa
  • Reactivity:
    Human
  • Form:
    Liquid
  • Gene ID:
    969
  • Antigen synonyms:
    EA1|MLR-3|member C|AIM|GP32/28|CLEC2C|CD69 antigen|C-type lectin domain family 2|BL-AC/P26
  • Storage buffer:
    PBS, 0.2% BSA, 0.1% gelatin, 0.09% sodium azide, pH 7.2
  • Storage temperature:
    4° C, store in dark, DO NOT FREEZE!
  • Concentration:
    5 µl/Test
  • Purification:
    Affinity chromatography
  • Size:
    100 Tests
  • Pk:
    100 Tests

Specifications

About this item

Description: The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets. Applications Reported: The FN50 antibody has been reported for use in flow cytometric analysis. Applications Tested: This FN50 antibody has been pre-titrated and tested by flow cytometric analysis of PHA-stimulated normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser Also called AIM or activation inducer molecule, CD69 is a gp28/34 disulfide bonded homodimer with a Mr of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride. The CD69 molecule is also present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen. It is involved in lymphocyte signal transduction.

Flow Cytometry: 5 µL (0.125 µg)/test

Type: Primary
Antigen: CD69
Clonality: Monoclonal
Clone: FN50
Conjugation: FITC (Fluorescein Isothiocyanate)
Epitope:
Host: Mouse
Isotype: IgG1, kappa
Reactivity: Human