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- Antibody type:Primary
- Antigen name:CD4 molecule
- Antigen symbol:CD4
- Clonality:Monoclonal
- Clone:GK1.5
- Conjugation:FITC (Fluorescein Isothiocyanate)
- Flow cytometry:Yes
- Host:Rat
- Isotype:IgG2b kappa
- Reactivity:Mouse
- Form:Liquid
- Gene ID:12504
- Antigen synonyms:CD4 receptor|T-cell surface antigen T4/Leu-3|T-cell surface glycoprotein CD4|CD4 antigen p55
- Storage buffer:PBS, 0.1% gelatin, 0.09% sodium azide, pH 7.2
- Storage temperature:4° C, store in dark, DO NOT FREEZE!
- Concentration:0.5 mg/mL
- Purification:Affinity chromatography
- Size:500 µg
- Pk:500 µG
Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5. Applications Reported: The GK1.5 antibody has been reported for use in flow cytometric analysis. Applications Tested: This GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
Flow Cytometry: 0.25 µg/test
Type: Primary
Antigen: CD4
Clonality: Monoclonal
Clone: GK1.5
Conjugation: FITC (Fluorescein Isothiocyanate)
Epitope:
Host: Rat
Isotype: IgG2b, kappa
Reactivity: Mouse