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Specifications
- 颗粒大小:90 µm
- 包装规格:50 ml
- 说明:Heparin Sepharose™ 6 Fast Flow
- pH 范围:4 - 12
- 基质:Cross-linked agarose, 6%, spherical
- 配体:Porcine heparin
- 存储温度:4 - 30 °C
- 配体密度:~4 mg heparin/ml
- 配体偶联法:Reductive Amination
- 流速:250 - 400 cm/h
- 类型:Liquid
Specifications
About this item
Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.
- For affinity chromatography
- Industrial purification of antithrombin III, coagulation factors and other plasma proteins
- Coupling chemistry gives chemically-stable ligand attachment
- Reductive amination coupling method
- Stability of medium solely dictated by heparin ligand
- BioProcess medium supported for industrial applications and well-established in approved processes
- Stable in commonly used aqueous buffers 0.1 M NaOH, 0.05 M sodium acetate, 8 M urea, 6 M guanidine hydrochloride, non-ionic detergents, 10% glycerol
Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin
This medium allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Additional applications include DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors.
Heparin Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Heparin Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand. Heparin Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.