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Specifications
- Flow rate:<4 ml/min
- Particle Size:34 µm
- Max. pressure:5 bar
- Capacity:1 ml
- Description:HiTrap™ IgM Purification HP
- Seq:01
- pH range:3 - 11
- Storage:4 to 8 °C, 20% Ethanol
- Column hardware:PP
- Bed dimensions:7×25 mm
- Pk:5
Specifications
About this item
HiTrap IgM Purification HP is a 1 ml column prepacked with Sepharose High Performance for fast and efficient purification of monoclonal IgM.
- Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture
- Prepacked with a thiophilic adsorption resin, 2-mercaptopyridine coupled to Sepharose High Performance
- Recovery of IgM is high with excellent activity retention
- The top and bottom frits are manufactured from porous polyethylene
Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.