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Affinity chromatography media, Benzamidine Sepharose™ 4 Fast Flow (High Sub)
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Specifications

  • Particle Size:
    90 µm
  • Pk:
    25 ml
  • Description:
    Benzamidine Sepharose™ 4 Fast Flow (High Sub)
  • Environmentally Preferable:
  • pH range:
    2 - 8
  • Matrix:
    Highly cross-linked 4% agarose
  • Ligand:
    p-aminobenzamidine [pABA]
  • Storage temperature:
    2 - 8 °C
  • Flow rate:
    30 - 300 cm/h
  • Type:
    Liquid

Specifications

About this item

Benzamidine Sepharose™ 4 Fast Flow (High Sub) is a tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as zymogens, including urokinase and prekallikrein.

  • For affinity chromatography
  • p-aminobenzamidine (pABA) immobilized on Sepharose 4 Fast Flow matrix gives high productivity, and allows easy scale-up
  • Effective removal of thrombin and factor Xa after tag cleavage of recombinant proteins
  • Amide coupling method
  • Stable in all commonly used aqueous buffers

Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates. Benzamidine Sepharose™ 4 Fast Flow (High Sub) is based on highly cross-linked 4% agarose enabling high flow rates without losing binding capacity.

For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.