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- Particle Size:90 µm
- Pk:200 mL
- Description:Phenyl Sepharose™ CL-4B
- pH range:3 - 12
- Matrix:Cross-linked agarose, 4%, spherical
- Ligand:Phenyl
- Storage temperature:4 - 30 °C
- Binding capacity:HSA (Mr 68 000) 15-20 mg/ml drained resin, ß-lactoglobulin (Mr 18 000) 3-5 mg/ml drained resin
- Ligand density:40 μmole/ml drained medium
- Flow rate:150 cm/h
- Type:Liquid
Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.
- Phenyl immobilized on 4% cross-linked agarose
- Aromatic ligand
- Stable in all commonly used aqueous buffers and additives such as 8 M urea and 6 M guanidine hydrochloride
- Negligible volume variation due to changes in pH or ionic strength
Octyl Sepharose® CL-4B and Phenyl Sepharose® CL-4B are covalently coupled to a cross-linked 4% agarose matrix by ether linkage, resulting in hydrophobic gels with minimal leakage and no ionic properties.