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规格
- Particle Size:90 µm
- Pk:25 ml
- Description:Lentil Lectin Sepharose™ 4B
- pH range:3 - 10
- Matrix:Agarose, 4%
- Ligand:Lentil lectin
- Storage temperature:2 - 8 °C
- Flow rate:<75 cm/h
- Type:Liquid
规格
关于此项目
Lentil lectin is a metalloprotein, containing Ca₂₊ and Mn₂₊, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars.
- For affinity chromatography
- Cyanogen bromide activation coupling methods
- Stable to all commonly used aqueous buffers
- Chelating agents should be avoided
- Negligible volume variation due to changes in pH or ionic strength
Lentil Lectin Sepharose 4B is a generally applicable group specific adsorbent and is routinely used in the preparation and purification of a number of glycoproteins and carbohydrate containing molecules. Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues. Compared to Con A, also for glycoprotein purification, Lentil Lectin distinguishes less sharply between glucosyl and mannosyl. Retains binding in 1% deoxycholate and is especially valuable for purification of detergent-solubilized membrane proteins. Chelating agents such as EDTA, 8 M urea or solutions having a pH below 3 should be avoided as these conditions result in removal of manganese from the lectin and loss of activity.