rProtein A Sepharose™ 4 Fast Flow is a well established antibody affinity medium is designed for the purification of monoclonal and polyclonal antibodies based on Sepharose™ Fast Flow platform. The recombinant protein A is produced in E.coli and engineered for an oriented coupling to giving a matrix with enhanced binding capacity. The epoxy-based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A and provides excellent purification in one step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose™ Fast Flow ideal for purification of monoclonal antibodies at both laboratory and process scale.
- For antibody affinity chromatography
- High dynamic binding capacity due to oriented, single-point attachment of protein A
- Well-established Protein A medium used in many approved MAb processes
- Epoxy activation coupling method
- No mammalian components involved during the manufacturing process
- Stable in all aqueous buffers commonly used in protein A chromatography - 10 mM HCI (pH 2), 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 6 M GuHCl, 20% ethanol
rProtein A Sepharose 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, rProtein A Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix. The recombinant protein A is produced in E. coli and has been specially engineered to favour an oriented coupling giving a matrix with enhanced binding capacity. The epoxy based coupling ensures low ligand leakage. The specificity of the recombinant protein A for the Fc region of IgG is similar to native protein A, giving a high purification factor in a single step. The high capacity, low ligand leakage and a well established base matrix makes rProtein A Sepharose 4 Fast Flow suitable for purification of monoclonal antibodies at both laboratory and process scale.
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- pH range:3 - 10
- Matrix:Cross-linked agarose, 4%, spherical
- Ligand:Recombinant protein A (E.coli)
- Storage temperature:2 - 8 °C
- Binding capacity:~50 mg hIgG/ml
- Ligand density:6 mg rProtein A/ml
- Ligand coupling method:Epoxy activation
- Flow rate:150 - 250 cm/h