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Specifications
- Particle Size:90 µm
- Pk:25 ml
- Description:NHS-Activated Sepharose™ 4 Fast Flow
- Seq:01
- Environmentally Preferable:
- pH range:3 - 13
- Matrix:4% cross-linked agarose
- Ligand:N-hydroxysuccinimide (NHS) activated
- Storage temperature:2 - 8 °C
- Flow rate:150 - 250 cm/h
- Type:Liquid
Specifications
About this item
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.
- For affinity chromatography
- NHS pre-activated medium for coupling of small amino-containing proteins and peptides in process-scale applications
- NHS coupling method results in chemically stable ligand attachment
- BioProcess medium supported for industrial applications
- High level of pre-activation gives a high degree of substitution of the selected ligand
- Stable in commonly used aqueous buffers provided that the ligand withstand the pH or the chemical environment
NHS-activated Sepharose 4 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, NHS-activated Sepharose 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support. This resin provides a spacer arm to the coupled ligand and is therefore particularly suitable for immobilising smaller protein and peptide ligands.
NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups.