Electrophoresis Reagents

Supplier: VWR Chemicals

VWR®, Fixing solution for isoelectric focusing in polyacrylamide ge

Supplier: VWR Chemicals

Destaining bags are designed to safely remove ethidium bromide from solution during overnight treatment. Each bag extracts up to 5 mg of ethidium bromide from solution, thus minimising the exposure of research personnel to this toxic material. The special absorbent mixture retains dye molecules in a convenient bag for removal and incineration.

Supplier: VWR Chemicals

EZ-Vision® 1 kb DNA ladder is a pre-mixture of DNA marker, loading buffer and a proprietary fluorescent DNA visualisation dye that can be applied directly to the gel. This non toxic, non mutagenic dye eliminates the need for ethidium bromide staining. The 1 kb ladder consists of 10 double-stranded bands with molecular weights between 200 and 10 000 bp. The intensity of individual bands varies to facilitate easy identification.

Supplier: VWR Chemicals

Ladder with 10 fragments ranging from 50 to 500 bp for easy band identification. Ready to use formula pre-mixed with loading dye.

Supplier: VWR Chemicals

PCR DNA Marker™ offers 8 fragments ranging from 50 to 2000 bp. The marker contains high intensity reference bands that are ideal for quick sizing of PCR products and restriction digests. Simply mix 10 μl of PCR DNA Marker™ with required quantity of loading buffer and load into well. There is sufficient material for 100 assays.

Supplier: VWR Chemicals

EZ-Vision® 100 bp DNA ladder is a pre-stained molecular weight ladder in loading buffer that allows immediate visualisation of DNA ladder post-electrophoresis.

Supplier: VWR Chemicals

VisiGlo™ and Visiglo Plus™ HRP chemiluminescent substrates are luminol-based chemiluminescent substrates designed for rapid and sensitive detection of peroxidase-labelled conjugates. Sensitivity ranges from picogram levels, captured by VisiGlo™ HRP chemiluminescent substrate, down to femtogram levels, exhibited by VisiGlo™ Prime and VisiGlo™ Select. VisiGlo™ Select displays the highest sensitivity, ideal for detecting low abundance proteins. The signal generated by substrate incubation is sustained between 8 to 10 hours allowing you to perform multiple exposures over an extended period of time.

Supplier: VWR Chemicals

DNA marker consists of 11 fragments ranging from 500 to 10000 bp for easy band identification.

Supplier: G BIOSCIENCES

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

Supplier: G BIOSCIENCES

The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.

Supplier: VWR Chemicals

High-Range DNA MW (molecular weight) marker offers 10 fragments ranging from 1503 to 48 502 bp. There is sufficient material for 75 to 150 assays.

Supplier: G BIOSCIENCES

G-Biosciences' Destain I contains approximately 45% methanol and 9% glacial acetic acid. G-Biosciences' Destain II contains approximately 5% methanol and 7% acetic acid.

Supplier: SIGMAALDRICH

SIGMAFAST™ BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium) tablets have been developed for use in immunochemistry as a precipitating substrate for the detection of alkaline phosphatase activity.

Supplier: GE HEALTHCARE

Rainbow™ molecular weight markers enable faster and simpler identification of proteins on SDS polyacrylamide gels. These ready to load markers provide sharper, more intense bands on gels and blots, and have improved band spacing for more accurate molecular weight determination. The bright, distinctive colours of the markers allow easier confirmation of transfer to blotting membranes and orientation.

Supplier: GE HEALTHCARE

The appearance of streaks that distort 2-D electrophoresis maps is a common problem, occurring most frequently when running gels that contain regions greater than pH 7,0. Increased sample load, increased length of the IPG strip, or using a narrower pH gradient worsens the problem. Extra spots on 2-D gels, caused by non specific oxidation of proteins, is another difficulty encountered when running gels containing basic regions. Both streaking and non specific oxidation result in poorly resolved protein patterns and reduced reproducibility between electrophoresis runs.

Supplier: GE HEALTHCARE

AlkPhos Direct™ Labelling and detection systems are based on the rapid, direct labelling of DNA or RNA probes with thermostable alkaline phosphatase. AlkPhos Direct™ combines the convenience of direct enzyme labelling (no blocking or antibody stages) with those of alkaline phosphatase detection (long light output and high sensitivity).