You searched for: Cloning Kits and Ligation
The aim of molecular cloning is to insert the gene of interest into a plasmid vector, which is then inserted into a cell that will express the protein encoded by the gene of interest. Once the protein is expressed, the protein function can be studied as it affects cell signaling, morphology, or other aspects. When considering a suitable cloning reagent, it is important to identify the cell type and culture conditions for the assay.
Random mutagenesis kits geneMorph II
Supplier: AGILENT
The GeneMorph II random mutagenesis kits have been formulated to produce a more uniform mutational spectrum when performing error prone PCR to achieve random mutagenesis.
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T4 DNA ligase
Supplier: GE HEALTHCARE
T4 DNA ligase catalyses the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction.
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T4 RNA ligase (from Escherichia coli)
Supplier: PROMEGA
T4 RNA ligase catalyses the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43,5 kDa. It also catalyses the addition of [5'-³²P] nucleoside 3',5'-bis (phosphate) onto single-stranded RNA.