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Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

Superose™ 12 Gel Filtration Media, Prep Grade

Superose™ 12 Gel Filtration Media, Prep Grade

Supplier: GE HEALTHCARE

Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability

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Ion exchange chromatography media, CM Sepharose™ Fast Flow

Ion exchange chromatography media, CM Sepharose™ Fast Flow

Supplier: GE HEALTHCARE

CM Sepharose™ Fast Flow is a weak cation exchanger based on the well established Sepharose™ Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

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Affinity chromatography media, MabSelect SuRe™

Affinity chromatography media, MabSelect SuRe™

Supplier: GE HEALTHCARE

MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.

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Ion exchange chromatography media, DEAE Sephacel™

Ion exchange chromatography media, DEAE Sephacel™

Supplier: GE HEALTHCARE

DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.

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SP Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva

SP Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva

Supplier: GE HEALTHCARE

SP Sephadex™ C-25 is a strong cation exchanger. The ion exchange group is a sulphopropyl group which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.

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HeLa protein digest standard, Pierce™

HeLa protein digest standard, Pierce™

Supplier: Thermo Fisher Scientific

The HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

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Ion exchange chromatography media, DEAE Sepharose™ Fast Flow

Ion exchange chromatography media, DEAE Sepharose™ Fast Flow

Supplier: GE HEALTHCARE

DEAE Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform. It is composed of cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. Scale-up with DEAE Sepharose™ Fast Flow is straight forward.

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Affinity chromatography media, Capto™ L

Affinity chromatography media, Capto™ L

Supplier: GE HEALTHCARE

Capto™ L is a bioprocessing chromatography resin for the capture of antibodies and antibody fragments. It combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein, L ligand, which has a strong affinity to the variable region of antibody kappa light chains. It is suitable for the capture of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

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Affinity coupling media, Epoxy-Activated Sepharose™ 6B

Supplier: GE HEALTHCARE

Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.

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Iodoacetamide (IAM), Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Iodoacetamide is a sulphhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterisation and peptide mapping.

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Protein digestion kits, trypsin kits, SMART Digest™

Supplier: Thermo Fisher Scientific

Thermo Scientific™ SMART Digest™ Trypsin Kits are designed to provide a simple to use, fast, highly reproducible and robust process for digestion of proteins in a high throughput format, meeting the needs of biopharmaceutical, bioanalytical and QA/QC environments.

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TCEP solution, neutral pH, Bond-Breaker™ Pierce™

TCEP solution, neutral pH, Bond-Breaker™ Pierce™

Supplier: Thermo Fisher Scientific

Bond-Breaker™ TCEP Solution (neutral pH) is a stable, 0,5 M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulphide bonds.

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