You searched for: Protein Purification
Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.
Gel filtration media, Sephadex™ G-25
Supplier: GE HEALTHCARE
Sephadex™ G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.
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Blue Sepharose™ 6 Fast Flow Affinity Chromatography Media, Cytiva
Supplier: GE HEALTHCARE
Blue Sepharose™ 6 Fast Flow is cibacron blue 3G coupled to Sepharose™ 6 Fast Flow. It is well established as an adsorbent for albumin and interferon at both laboratory and process scale. The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.
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Sepharose™ CL-4B Phenyl Hydrophobic Interaction Chromatography Media
Supplier: GE HEALTHCARE
Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.
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Ion exchange chromatography media, SOURCE™ 30Q
Supplier: GE HEALTHCARE
SOURCE™ 30Q is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.
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Affinity coupling media, CNBr-Activated Sepharose™ 4B
Supplier: GE HEALTHCARE
CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.
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Protein G Sepharose™ 4 Fast Flow Affinity Chromatography Media
Supplier: GE HEALTHCARE
Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.
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DEAE Sephadex™ A-50 Ion Exchange Chromatography Media, Cytiva
Supplier: GE HEALTHCARE
DEAE Sephadex™ A-25 and DEAE Sephadex™ A-50 are weak anion exchangers. Sephadex™ ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.
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Butyl-S Sepharose™ 6 Fast Flow Hydrophobic interaction chromatography media
Supplier: GE HEALTHCARE
Butyl-S Sepharose™ 6 Fast Flow is composed of cross-linked 6% agarose beads modified with butyl groups via a thio-ether linkage. This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations.
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Sepharose™ CL-2B Gel Filtration Base Matrix
Supplier: GE HEALTHCARE
Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
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Affinity coupling media, NHS-Activated Sepharose™ 4 Fast Flow
Supplier: GE HEALTHCARE
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.
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Affinity purification media, GammaBind™ Plus Sepharose™
Supplier: GE HEALTHCARE
GammaBind™ Plus Sepharose™ is GammaBind G, type 3, covalently immobilised to Sepharose™ CL-6B by malimide linkage.
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Hydrophobic interaction chromatography media, Phenyl Sepharose™ High Performance
Supplier: GE HEALTHCARE
Phenyl Sepharose™ High Performance is an aromatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority.
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Ion exchange chromatography media, DEAE Sepharose™ CL-6B
Supplier: GE HEALTHCARE
DEAE Sepharose™ CL-6B is a weak anion exchanger.
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Phenyl Sepharose™ 6 Fast Flow (Low Sub) Hydrophobic Interaction Chromatography Media
Supplier: GE HEALTHCARE
Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity
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CM Sephadex™ C-50 Ion Exchange Chromatography Media, Cytiva
Supplier: GE HEALTHCARE
Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.
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QAE Sephadex™ Ion Exchange Chromatography Media, Cytiva
Supplier: GE HEALTHCARE
Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.
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Affinity chromatography media, MabSelect™ PrismA
Supplier: GE HEALTHCARE
MabSelect™ PrismA has been improved with an optimised high-flow agarose base matrix and a genetically engineered protein A-derived ligand, allowing future demands in mAb processing to be met.
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Ion exchange chromatography media, SOURCE™ 15S
Supplier: GE HEALTHCARE
SOURCE™ 15S is a synthetic high performance, preparative, chromatography medium, based on a 15 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.
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Gel filtration media, Sephadex™ G-75
Supplier: GE HEALTHCARE
Sephadex™ G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.
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Superose™ 12 Gel Filtration Media, Prep Grade
Supplier: GE HEALTHCARE
Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability
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SP Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva
Supplier: GE HEALTHCARE
SP Sephadex™ C-25 is a strong cation exchanger. The ion exchange group is a sulphopropyl group which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.
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Ion exchange chromatography media, DEAE Sepharose™ Fast Flow
Supplier: GE HEALTHCARE
DEAE Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform. It is composed of cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. Scale-up with DEAE Sepharose™ Fast Flow is straight forward.
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Affinity chromatography media, Capto™ L
Supplier: GE HEALTHCARE
Capto™ L is a bioprocessing chromatography resin for the capture of antibodies and antibody fragments. It combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein, L ligand, which has a strong affinity to the variable region of antibody kappa light chains. It is suitable for the capture of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).
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TCEP solution, neutral pH, Bond-Breaker™ Pierce™
Supplier: Thermo Fisher Scientific
Bond-Breaker™ TCEP Solution (neutral pH) is a stable, 0,5 M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulphide bonds.
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Plasmid purification media, PlasmidSelect Xtra
Supplier: GE HEALTHCARE
PlasmidSelect Xtra is a thiophilic aromatic adsorption chromatography medium with a selectivity that allows generic purification of supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms.
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Affinity chromatography media, Benzamidine Sepharose™ 4 Fast Flow (High Sub)
Supplier: GE HEALTHCARE
Benzamidine Sepharose™ 4 Fast Flow (High Sub) is a tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as zymogens, including urokinase and prekallikrein.
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Affinity chromatography media, MabSelect™
Supplier: GE HEALTHCARE
MabSelect™ is a protein A affinity resin designed for capturing monoclonal antibodies (mAbs) from large sample volumes.
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Con A Sepharose™ 4B Affinity Chromatography Media, Cytiva
Supplier: GE HEALTHCARE
Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method.
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CM Sephadex™ C-25 Ion Exchange Chromatography Media
Supplier: GE HEALTHCARE
Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.
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Gel filtration media, Superdex™ 30 PG
Supplier: GE HEALTHCARE
Superdex prep grade is a preparative gel filtration medium with a composite matrix of dextran and agarose