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Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

Affinity chromatography media, Chelating Sepharose™ Fast Flow

Affinity chromatography media, Chelating Sepharose™ Fast Flow

Supplier: GE HEALTHCARE

Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.

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Sepharose™ CL-4B Gel Filtration Base Matrix

Sepharose™ CL-4B Gel Filtration Base Matrix

Supplier: GE HEALTHCARE

Sepharose™ CL-4B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

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Gel filtration media, Sephadex™ LH-20

Gel filtration media, Sephadex™ LH-20

Supplier: GE HEALTHCARE

Sephadex™ LH-20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid and low molecular weight peptides. Sephadex™ LH-20 is pre-pared by hydroxypropylation of Sephadex™ G-25, a bead-formed dextran medium.

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HeLa protein digest standard, Pierce™

HeLa protein digest standard, Pierce™

Supplier: Thermo Fisher Scientific

The HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

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Affinity purification media, GammaBind™ Plus Sepharose™

Supplier: GE HEALTHCARE

GammaBind™ Plus Sepharose™ is GammaBind G, type 3, covalently immobilised to Sepharose™ CL-6B by malimide linkage.

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Hydrophobic interaction chromatography media, Phenyl Sepharose™ High Performance

Hydrophobic interaction chromatography media, Phenyl Sepharose™ High Performance

Supplier: GE HEALTHCARE

Phenyl Sepharose™ High Performance is an aromatic hydrophobic interaction chromatography (HIC) medium, designed for intermediate and polishing step purification steps when high resolution has priority.

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Ion exchange chromatography media, DEAE Sepharose™ CL-6B

Ion exchange chromatography media, DEAE Sepharose™ CL-6B

Supplier: GE HEALTHCARE

DEAE Sepharose™ CL-6B is a weak anion exchanger.

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Phenyl Sepharose™ 6 Fast Flow (Low Sub) Hydrophobic Interaction Chromatography Media

Phenyl Sepharose™ 6 Fast Flow (Low Sub) Hydrophobic Interaction Chromatography Media

Supplier: GE HEALTHCARE

Phenyl Sepharose 6 Fast Flow is a well established, standard aromatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification requiring low to resin hydrophobicity

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CM Sephadex™ C-50 Ion Exchange Chromatography Media, Cytiva

CM Sephadex™ C-50 Ion Exchange Chromatography Media, Cytiva

Supplier: GE HEALTHCARE

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

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QAE Sephadex™ Ion Exchange Chromatography Media, Cytiva

QAE Sephadex™ Ion Exchange Chromatography Media, Cytiva

Supplier: GE HEALTHCARE

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

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Affinity chromatography media, MabSelect™ PrismA

Affinity chromatography media, MabSelect™ PrismA

Supplier: GE HEALTHCARE

MabSelect™ PrismA has been improved with an optimised high-flow agarose base matrix and a genetically engineered protein A-derived ligand, allowing future demands in mAb processing to be met.

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Ion exchange chromatography media, SOURCE™ 15S

Ion exchange chromatography media, SOURCE™ 15S

Supplier: GE HEALTHCARE

SOURCE™ 15S is a synthetic high performance, preparative, chromatography medium, based on a 15 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.

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Gel filtration media, Sephadex™ G-75

Gel filtration media, Sephadex™ G-75

Supplier: GE HEALTHCARE

Sephadex™ G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

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Superose™ 12 Gel Filtration Media, Prep Grade

Superose™ 12 Gel Filtration Media, Prep Grade

Supplier: GE HEALTHCARE

Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability

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SP Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva

SP Sephadex™ C-25 Ion Exchange Chromatography Media, Cytiva

Supplier: GE HEALTHCARE

SP Sephadex™ C-25 is a strong cation exchanger. The ion exchange group is a sulphopropyl group which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.

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Ion exchange chromatography media, DEAE Sepharose™ Fast Flow

Ion exchange chromatography media, DEAE Sepharose™ Fast Flow

Supplier: GE HEALTHCARE

DEAE Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform. It is composed of cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. Scale-up with DEAE Sepharose™ Fast Flow is straight forward.

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Affinity chromatography media, Capto™ L

Affinity chromatography media, Capto™ L

Supplier: GE HEALTHCARE

Capto™ L is a bioprocessing chromatography resin for the capture of antibodies and antibody fragments. It combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein, L ligand, which has a strong affinity to the variable region of antibody kappa light chains. It is suitable for the capture of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

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TCEP solution, neutral pH, Bond-Breaker™ Pierce™

TCEP solution, neutral pH, Bond-Breaker™ Pierce™

Supplier: Thermo Fisher Scientific

Bond-Breaker™ TCEP Solution (neutral pH) is a stable, 0,5 M solution of the thiol-free, phosphine-based TCEP compound, useful as a 10X stock for addition to SDS-PAGE sample loading buffers to reduce protein disulphide bonds.

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SDS-PAGE Clean Up Kit

SDS-PAGE Clean Up Kit

Supplier: GE HEALTHCARE

SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.

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Superose™ 6 Gel Filtration Media, Prep Grade

Superose™ 6 Gel Filtration Media, Prep Grade

Supplier: GE HEALTHCARE

Superose™ 6 PG for separations of proteins and other biomolecules according to size. Wide separation in two molecular weight ranges from 1000 up to 5 million.

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SP Sepharose™ XL Ion Exchange Chromatography Media

SP Sepharose™ XL Ion Exchange Chromatography Media

Supplier: GE HEALTHCARE

SP Sepharose™ XL is a strong cation exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.

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Ion exchange chromatography media, SOURCE™ 15Q

Ion exchange chromatography media, SOURCE™ 15Q

Supplier: GE HEALTHCARE

Mono-sized 15 um rigid beads gives low back pressure and high flow rates allowing for high productivity.

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Affinity chromatography media, MabSelect SuRe™ LX

Affinity chromatography media, MabSelect SuRe™ LX

Supplier: GE HEALTHCARE

MabSelect SuRe™ LX is a protein A affinity medium with very high dynamic binding capacity at extended residence times and is developed for high titer antibody processes.

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Affinity coupling media, CNBr-activated Sepharose™ 4

Affinity coupling media, CNBr-activated Sepharose™ 4

Supplier: GE HEALTHCARE

CNBr-activated Sepharose™ 4 Fast Flow is based on the Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide.

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Gel filtration media, Sephadex™ G-100

Gel filtration media, Sephadex™ G-100

Supplier: GE HEALTHCARE

Sephadex™ G-100 is useful for desalting and buffer exchange of very large biomolecules. Sephadex™ G-100 Superfine is designed for specific cases requiring very high resolution.

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Ion exchange chromatography media, CM Sepharose™ Fast Flow

Ion exchange chromatography media, CM Sepharose™ Fast Flow

Supplier: GE HEALTHCARE

CM Sepharose™ Fast Flow is a weak cation exchanger based on the well established Sepharose™ Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

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Gel filtration media, Sephadex™ G-25

Gel filtration media, Sephadex™ G-25

Supplier: GE HEALTHCARE

Sephadex™ G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.

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Ion exchange chromatography media, SOURCE™ 30Q

Ion exchange chromatography media, SOURCE™ 30Q

Supplier: GE HEALTHCARE

SOURCE™ 30Q is a synthetic high performance, preparative, chromatography medium, based on a 30 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.

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Sepharose™ CL-4B Phenyl Hydrophobic Interaction Chromatography Media

Sepharose™ CL-4B Phenyl Hydrophobic Interaction Chromatography Media

Supplier: GE HEALTHCARE

Octyl-Sepharose™ CL-4B is a well-proven medium for hydrophobic interaction chromatography (HIC). Octyl immobilised on 4% cross-linked agarose.

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Iodoacetamide (IAM), Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Iodoacetamide is a sulphhydryl-reactive alkylating reagent used to block reduced cysteine residues for protein characterisation and peptide mapping.

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