Ion exchange chromatography media, DEAE Sephacel™
Supplier: GE HEALTHCARE
DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.
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Affinity chromatography media, MabSelect SuRe™
Supplier: GE HEALTHCARE
MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.
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Affinity coupling media, Epoxy-Activated Sepharose™ 6B
Supplier: GE HEALTHCARE
Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.