You searched for: Cellular Assays
Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
Triglyceride colorimetric assay kit
Supplier: CAYMAN
Triglyceride Colorimetric Assay Kit is a simple, reproducible and sensitive tool for assaying triglycerides in plasma, serum, cell lysates and tissue homogenate samples.
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ATP assay system, ENLITEN®
Supplier: PROMEGA
The ENLITEN® ATP Assay System Bioluminescence Detection Kit is designed for the rapid and quantitative measurement of adenosine 5´-triphosphate (ATP) levels for the indirect detection of biocontamination.
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CellTiter 96® AQueous MTS Reagent Powder
Supplier: PROMEGA
CellTiter 96® AQueous MTS Reagent Powder is a novel tetrazolium compound used in colorimetric assays to determine the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.
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Luciferase assay system, ONE-Glo™
Supplier: PROMEGA
The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells in 96- and 384-well plates.
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Luciferase assay system, Nano-Glo®
Supplier: PROMEGA
The Nano-Glo® Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc® luciferase with a half-life of approximately 120 minutes in commonly used tissue culture media.
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Luciferase assay system, Bio-Glo™
Supplier: PROMEGA
The Bio-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous reagent for the detection of firefly luciferase reporter gene expression in the ADCC Reporter Bioassay.
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Caspase-3 assay kits, NucView™ 488
Supplier: BIOTIUM
NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.
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Griess reagent system
Supplier: PROMEGA
The Griess Reagent System measures nitrite (NO²⁻), which is one of two primary stable and non volatile breakdown products of nitric oxide (NO).
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CellTiter 96® AQueous non-radioactive cell proliferation assay (MTS)
Supplier: PROMEGA
The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay is a homogeneous, colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.
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CytoTox 96® Non-Radioactive Cytotoxicity Assay
Supplier: PROMEGA
The CytoTox 96® Non-Radioactive Cytotoxicity Assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [⁵¹Cr] is released in radioactive assays.
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Cell viability assay, luminescent, CellTiter-Glo®
Supplier: PROMEGA
The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. It is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays.
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Cell proliferation assays, CellTiter 96® AQueous One Solution
Supplier: PROMEGA
The CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.
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CellTiter-Glo® 3D cell viability assay
Supplier: PROMEGA
The CellTiter-Glo® 3D Cell Viability Assay is designed for determining cell viability in 3D microtissue spheroids. The assay reagent penetrates large spheroids and has increased lytic capacity allowing more accurate determination of viability compared to other assay methods.
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Dual-Luciferase® reporter (DLR™) assay system
Supplier: PROMEGA
The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample.
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Glutathione assay, GSH-Glo™
Supplier: PROMEGA
GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH) in cells or in various biological samples. The assay is based on the conversion of a luciferin derivative into luciferin in the presence of GSH.
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Luciferase assay systems
Supplier: PROMEGA
The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 to 20 moles of luciferase can be measured under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) assay.
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Luciferase assay system, Dual-Glo®
Supplier: PROMEGA
The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantisation of a stable luminescent signal from two reporter genes in a single sample. The system is optimised for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media.
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Oxidative stress detection kit, ROS-Glo™ H₂O₂ assay
Supplier: PROMEGA
The ROS-Glo™ H₂O₂ Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H₂O₂), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions. A derivatised luciferin substrate is incubated with sample and reacts directly with H₂O₂ to generate a luciferin precursor. The ROS-Glo™ detection solution converts the precursor to luciferin and provides Ultra-Glo™ Recombinant Luciferase to produce light signal that is proportional to the level of H₂O₂ present in the sample.
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Oxidative stress assays, GSH/GSSG-Glo™ assay
Supplier: PROMEGA
The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells. A change in GSH levels is important in the assessment of toxicological responses and is an indicator of oxidative stress, potentially leading to apoptosis or cell death. The assay provides a simple, rapid multiwell-plate format where stable luminescent signals are correlated with either the total GSH or the GSSG concentration of a sample directly in culture wells.
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Luciferase assay system, Steady-Glo®
Supplier: PROMEGA
High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System is designed for this purpose of providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media, and does not require sample preprocessing. Throughput rates of several thousand samples per hour may be achieved with high reproducibility under standard laboratory conditions.