You searched for: Assays

Supplier: G BIOSCIENCES

A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.

Supplier: G BIOSCIENCES

CB™ Protein Assay, a Coomassie dye based protein assay based on the Bradford Protein Assay, is suitable for the simple and rapid estimation of protein concentration. The binding of protein to the dye results in a colour change from brown to blue. The change in colour density is proportional to protein concentration. Protein estimation can be performed using as little as 0,5 µg protein.

Supplier: CAYMAN

Triglyceride Colorimetric Assay Kit is a simple, reproducible and sensitive tool for assaying triglycerides in plasma, serum, cell lysates and tissue homogenate samples.

Supplier: PROMEGA

The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample.

Supplier: Thermo Fisher Scientific

The bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. The Pierce™ coomassie plus protein assay is a ready-to-use, reducing agent-compatible, improved bradford assay reagent to quickly measure (A595 nm) total protein concentration compared to a protein standard.

Supplier: PROMEGA

The CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.

Supplier: PROMEGA

The ENLITEN® ATP Assay System Bioluminescence Detection Kit is designed for the rapid and quantitative measurement of adenosine 5´-triphosphate (ATP) levels for the indirect detection of biocontamination.

Supplier: PROMEGA

The Bio-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous reagent for the detection of firefly luciferase reporter gene expression in the ADCC Reporter Bioassay.

Supplier: PROMEGA

The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. It is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays.

Supplier: PROMEGA

GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH) in cells or in various biological samples. The assay is based on the conversion of a luciferin derivative into luciferin in the presence of GSH.

Supplier: PROMEGA

The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells in 96- and 384-well plates.

Supplier: Thermo Fisher Scientific

Micro BCA™ protein assay kit is a specialised version of Pierce™ BCA protein assay for determining the protein concentration of dilute samples.

Supplier: PROMEGA

The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantisation of a stable luminescent signal from two reporter genes in a single sample. The system is optimised for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media.

Supplier: Thermo Fisher Scientific

Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Supplier: Thermo Fisher Scientific

The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.

Supplier: PROMEGA

The Nano-Glo® Luciferase Assay System provides a simple, single-addition reagent that generates a glow-type signal in the presence of NanoLuc® luciferase with a half-life of approximately 120 minutes in commonly used tissue culture media.

Supplier: PROMEGA

The ROS-Glo™ H₂O₂ Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H₂O₂), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions. A derivatised luciferin substrate is incubated with sample and reacts directly with H₂O₂ to generate a luciferin precursor. The ROS-Glo™ detection solution converts the precursor to luciferin and provides Ultra-Glo™ Recombinant Luciferase to produce light signal that is proportional to the level of H₂O₂ present in the sample.

Supplier: PROMEGA

CellTiter 96® AQueous MTS Reagent Powder is a novel tetrazolium compound used in colorimetric assays to determine the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.

Supplier: PROMEGA

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System is designed for this purpose of providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media, and does not require sample preprocessing. Throughput rates of several thousand samples per hour may be achieved with high reproducibility under standard laboratory conditions.

Supplier: PROMEGA

The CellTiter-Glo® 3D Cell Viability Assay is designed for determining cell viability in 3D microtissue spheroids. The assay reagent penetrates large spheroids and has increased lytic capacity allowing more accurate determination of viability compared to other assay methods.

Supplier: PROMEGA

The CytoTox 96® Non-Radioactive Cytotoxicity Assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [⁵¹Cr] is released in radioactive assays.

Supplier: PROMEGA

The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay is a homogeneous, colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.

Supplier: BIOTIUM

NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.

Supplier: PROMEGA

The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 to 20 moles of luciferase can be measured under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) assay.

Supplier: PROMEGA

The Griess Reagent System measures nitrite (NO²⁻), which is one of two primary stable and non volatile breakdown products of nitric oxide (NO).

Supplier: PROMEGA

The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells. A change in GSH levels is important in the assessment of toxicological responses and is an indicator of oxidative stress, potentially leading to apoptosis or cell death. The assay provides a simple, rapid multiwell-plate format where stable luminescent signals are correlated with either the total GSH or the GSSG concentration of a sample directly in culture wells.

Supplier: GE HEALTHCARE

The Cell Proliferation Kit is an ICC system for monitoring cell proliferation using monoclonal anti-5-bromo-2'-deoxyuridine (BrdU) to detect BrdU incorporated into cellular DNA.

Supplier: GE HEALTHCARE

RAPD reactions are pre-optimized for use with a wide variety of organisms.

Supplier: MIRUS

Label IT® nucleic acid labeling kits are suitable for direct non-enzymatic labeling of DNA and RNA. Permanent, non-destructive modification of nucleic acid residues is ideal for many diverse applications.

Supplier: GE HEALTHCARE

The Amerlex-M system is a magnetic separation system for use in immunoassay. It combines the advantages of second antibody solid phase separation with liquid phase kinetics. Its use eliminates centrifugation during the separation of free and bound ligand. Amerlex-M donkey anti-rabbit reagent consists of magnetisable polymer beads coated with second antibody. The use of this reagent enables phase separation by introduction of a magnetic field. It is used where the primary antibody is of rabbit origin and is particularly suitable for iodine-125 radioimmunoassays as bound counts end up in the pellet.