Vectors, Plasmids and Libraries
In molecular cloning, vectors are DNA molecules used to artificially deliver foreign genetic material into another cell. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes, with the most commonly used of these being plasmids. Vectors may be used for cloning, and certain vectors are specifically tailored to that exact purpose, while others may be designed specifically for transcription and protein expression. Simpler transcription vectors are used to amplify their inserted genetic material.
E. coli BL21
Supplier: GE HEALTHCARE
The E. coli BL21 strain is protease-deficient for optimal expression of a variety of recombinant proteins.
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M13KO7 Helper Phage, Cytiva
Supplier: GE HEALTHCARE
M13 phage for the GST expression system.
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Polyadenylic acid (poly-A)
Supplier: GE HEALTHCARE
Poly(A) (polyadenylic acid) can be used to evaluate the binding of potential therapeutic agents to mRNA in drug development research.
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Expression vectors, pGEX
Supplier: GE HEALTHCARE
The pGEX vectors have an expanded multiple cloning site (MCS) that contains six restriction sites. The expanded MCS facilitates the unidirectional cloning of cDNA inserts obtained from libraries constructed using many available lambda vectors. pGEX-6P-1, pGEX-6P-2, and pGEX-6P-3 each encode the recognition sequence for site-specific cleavage by PreScission Protease between the GST domain and the multiple cloning site. pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3 are derived from pGEX-2T and contain a Thrombin recognition site. pGEX-5X-1, pGEX-5X-2, and pGEX5X-3 are derivatives of pGEX-3X and possess a Factor Xa recognition site.