Enzymes
Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.
VWR® Penicillinase (beta-Lactamase)
Supplier: VWR Chemicals
A freeze-dried product with >3000 ß-l units per vial.
One unit of ß-l activity is defined as the amount of enzyme that will catalyse the hydrolysis of 1,0 µm of benzylpenicillin per minute at 25 °C and pH 7,0.
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J.T.Baker® Endonuclease
Supplier: Avantor
J.T.Baker® Endonuclease Biotech Reagent meets the strictest cGMP standards and is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Biotech Reagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease Biotech Reagent is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. Absence of proteolytic activity. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Biotech Reagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.
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J.T.Baker® Endonuclease
Supplier: Avantor
J.T.Baker® Endonuclease Ultrapure Bioreagent is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Ultrapure Bioreagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Ultrapure Bioreagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.
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VWR® Trichoderma viride Cellulase
Supplier: VWR Chemicals
10,000 units is that quantity of enzyme which will degrade 2 cm² of cellulose filter paper (17 mg) in 1 minute in 0,1 M acetate buffer at pH 4,0.
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VWR®, Pronase E
Supplier: VWR Chemicals
Off-white to light brown powder from Streptomyces griseus.
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Pepsin
Supplier: Thermo Fisher Scientific
Contains either Lactose or Maltodextrin excipient
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illustra Exonuclease I
Supplier: GE HEALTHCARE
Exonuclease I acts specifically on single-stranded DNA degrading it processively in the 3'- to 5'-direction, producing 5'-mononucleotides. Applications include eliminating residual single-stranded DNA containing a 3'-terminus, measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA, measuring DNA helicase activity.
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Trypsin
Supplier: PROMEGA
Immobilised trypsin provides a fast and convenient method for digesting a range of concentrations of purified protein or complex protein mixtures. Digested peptides are easily separated from the immobilised trypsin as they flow through the spin column into the collection tube. Immobilised trypsin is easily removed from the peptide solution because the trypsin does not pass though the column frit.
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Staphylococcus aureus (Strain V8) protease V8, MP Biomedicals
Supplier: MP BIOMEDICALS
Protease V8 specifically cleaves peptide bonds on the COOH terminal side of either aspartic or glutamic acids. In the presence of ammonium, the specificity is limited to glutamic sites.