You searched for: Enzymes
Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.
VWR® Trichoderma viride Cellulase
Supplier: VWR Chemicals
10,000 units is that quantity of enzyme which will degrade 2 cm² of cellulose filter paper (17 mg) in 1 minute in 0,1 M acetate buffer at pH 4,0.
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VWR® Penicillinase (beta-Lactamase)
Supplier: VWR Chemicals
A freeze-dried product with >3000 ß-l units per vial.
One unit of ß-l activity is defined as the amount of enzyme that will catalyse the hydrolysis of 1,0 µm of benzylpenicillin per minute at 25 °C and pH 7,0.
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VWR®, Cod Uracil-DNA glycosylase
Supplier: VWR Chemicals
Uracil-DNA glycosylase (UNG), cod is a thermolabile recombinant enzyme produced in E. coli (ung-) using a modified ung gene derived from Atlantic cod. It degrades uracil-containing single- and double-stranded DNA, but not RNA or thymidine-containing DNA, by hydrolysing the N-glycosidic bond between deoxyribose sugar and the base in uracil. This generates alkaline-sensitive apyramidinic sites in the DNA that will be cleaved upon a combination of alkaline conditions and high temperature.
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J.T.Baker® Endonuclease
Supplier: Avantor
J.T.Baker® Endonuclease Ultrapure Bioreagent is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Ultrapure Bioreagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Ultrapure Bioreagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.
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J.T.Baker® Endonuclease
Supplier: Avantor
J.T.Baker® Endonuclease Biotech Reagent meets the strictest cGMP standards and is designed for the degradation of both single stranded and double stranded DNA and RNA. J.T.Baker® Endonuclease Biotech Reagent is used to ensure host cell DNA impurities are removed; driving process efficiency by lowering viscosity and preventing aggregation. J.T.Baker® Endonuclease Biotech Reagent is an enzyme based upon the native endonuclease of Serratia marcescens, enabling rapid clearance of residual DNA and RNA during the production and purification of both recombinant proteins and viral vectors. Non-animal origin. Absence of proteolytic activity. The purity of materials in non-negotiable. J.T.Baker® Endonuclease Biotech Reagent acts to degrade and eliminate extraneous genetic material, ensuring the pristine quality of your final product.
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VWR®, HL-dsDNase
Supplier: VWR Chemicals
VWR® Life Science's DNase, Double-Strand Specific, Heat-Labile is a recombinant endonuclease that cleaves phosphodiester bonds in DNA to yield 2 to 8 bp oligonucleotides with 5’-phosphate and 3’-hydroxyl termini.
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VWR®, Ficin
Supplier: VWR Chemicals
Source is Fig Latex. Fine, light beige powder
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VWR®, Bovine Ribonuclease A (from Pancreas)
Supplier: VWR Chemicals
Ribonuclease A is an endoribonuclease that efficiently hydrolyses RNA from tissue or bacterial cell cultures.
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VWR®, Pronase E
Supplier: VWR Chemicals
Off-white to light brown powder from Streptomyces griseus.
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DNA modification enzymes
Supplier: GE HEALTHCARE
A high specific activity, heat-labile alkaline phosphatase. Completely and irreversibly inactivated in Tris buffers at pH 8,0 to 8,5 by heating for 15 minutes at 65 °C. No further treatment is necessary.
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IdeS Protease
Supplier: PROMEGA
IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). IdeZ Protease is an immunoglobulin-degrading enzyme from Streptococcus equi subspecies zooepidemicus.
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Thermolysin
Supplier: PROMEGA
Thermolysin is a thermostable metalloproteinase. The high digestion temperature stability of this enzyme may be useful as an alternative to denaturants to improve digestion of proteolytically resistant proteins.
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Ascorbate oxidase (from Cucumis sp.)
Supplier: SIGMAALDRICH
Ascorbate Oxidase is an enzyme belonging to the family of oxidoreductases that catalyses the reaction: Ascorbic acid + ½ O₂ ascorbate oxidase → Dehydroascorbic acid + H₂O.
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Aldehyde dehydrogenase (from Yeast)
Supplier: SIGMAALDRICH
Aldehyde dehydrogenase from baker′s yeast catalyses the reduction of pyridine nucleotides by several aldehydes. It catalyses the oxidation of a wide range of substrates, such as acetaldehyde, formaldehyde, propionaldehyde, n-butylaldehyde, isobutylaldehyde, n-valeraldehyde, caproaldehyde, benzaldehyde, glycoaldehyde, D-glyceraldehyde, malonic semialdehyde, and succinic aldehyde. The enzyme has been used to study the production of ethanol and isobutanol.
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Pepsin
Supplier: Thermo Fisher Scientific
Contains either Lactose or Maltodextrin excipient
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illustra Exonuclease I
Supplier: GE HEALTHCARE
Exonuclease I acts specifically on single-stranded DNA degrading it processively in the 3'- to 5'-direction, producing 5'-mononucleotides. Applications include eliminating residual single-stranded DNA containing a 3'-terminus, measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA, measuring DNA helicase activity.
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Trypsin
Supplier: PROMEGA
Immobilised trypsin provides a fast and convenient method for digesting a range of concentrations of purified protein or complex protein mixtures. Digested peptides are easily separated from the immobilised trypsin as they flow through the spin column into the collection tube. Immobilised trypsin is easily removed from the peptide solution because the trypsin does not pass though the column frit.
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Bovine hyaluronidase (from Testes)
Supplier: TCI
Bovine hyaluronidase (from Testes)
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Thrombin
Supplier: GE HEALTHCARE
Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).