Cell Culture Supplements and Additives

Supplier: VWR Chemicals

Contains a mixture of protease inhibitors: AEBSF, Aprotinin, E-64, Bestatin and Leupeptin. Each vial can be reconstituted in 1 ml deionised water to form a 100X solution.

Supplier: ABCAM

Mono AEC/Plus is a single, highly stable AEC chromogen/substrate working solution.

Supplier: CYTIVA HYCLONE

Supplements cell culture with concentrated amino acids. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: CYTIVA HYCLONE

Supplements cell culture with amino acids, vitamins, salts, trace elements, and glucose. Manufactured to meet cGMP manufacturing standards and QC specifications.

Supplier: GE HEALTHCARE

Sample preparation often requires the inhibition of protease activity. Protease inhibitor mix combines competitive and non competitive protease inhibitors, which protect proteins from proteolysis during purification from animal tissues, plant tissues, yeast and bacteria.

Supplier: Corning

An in vitro system for assessing the invasive potential of both malignant and normal cells.

Supplier: GE HEALTHCARE

The double-stranded polyribonucleotide poly(I)·poly(C) induces interferon in rabbit cells, and there has been interest in its effectiveness for inducing human interferon in vitro.

Supplier: Thermo Fisher Scientific

Phosphorylation and dephosphorylation is a molecular on/off switch that regulates a number of key biological pathways within the cell, including signal transduction, cell division, and apoptosis. The Halt Phosphatase Inhibitor Cocktail preserves phosphorylation of proteins in cell lysates and tissue extracts.

Supplier: Thermo Fisher Scientific

Halt protease and phosphatase inhibitor cocktail provides the convenience of a single solution with full sample protection. This broad-spectrum inhibitor cocktail is specifically optimised to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases.

Supplier: Corning

Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.

Supplier: Corning

Corning® Cell Recovery Solution allows for the recovery of cells cultured on Corning® Matrigel® Basement Membrane Matrix for subsequent biochemical analysis. Corning® Cell Recovery Solution depolymerises Corning® Matrigel® Matrix gels without enzymatic digests and lengthy incubation periods at high temperatures. Cells are released without damage thereby avoiding biochemical changes during incubation and digestion of extracellular portions of cell-surface receptors and adhesion molecules.

Supplier: Corning

Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.

Supplier: Corning

Endothelial Cell Growth Supplement (ECGS) is a broadly used supplement to culture a variety of cells, particularly endothelial cells.

Supplier: Thermo Fisher Scientific

Halt Protease Inhibitor Cocktail provides all the protection of a broad-spectrum protease inhibitor in a single, ready-to-use liquid format. Halt protects against protein degradation by serine, cysteine, and aspartic acid proteases and aminopeptidases. Metalloproteases are inhibited by the optional use of 0,5 M EDTA. The 100X Halt stock solution makes it easy to dispense the required amount (10 µl per 1 ml) of protease inhibitors into buffers or lysis reagents prior to extraction of proteins from cultured cells, animal tissues, plant tissues, yeast or bacteria. Halt Protease Inhibitor Cocktails are compatible with Thermo Scientific Pierce Protein Extraction Reagents. Halt EDTA-free formulation is ideal for preparing samples that will be analysed by 2-D gel electrophoresis, immobilised metal chelate affinity chromatography (IMAC) and other downstream application where chelating agents interfere.

Supplier: Corning

Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.

Supplier: Corning

Corning® Cell-Tak™ Cell and Tissue Adhesive is a formulation of polyphenolic proteins extracted from Mytilus edulis (marine mussel). These proteins are the key components of the glue secreted by the mussel to anchor itself to solid substrates in the marine environment. Corning® Cell-Tak™ is used to attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, PTFE, and biological materials. It can simplify the manipulation of biological samples for a number of in vitro techniques, including in situ hybridisation, immunoassays, microinjection, immunohistochemistry, and establishing primary cells in culture. Corning® Cell-Tak™ is biocompatible and demonstrates no species specificity.