"derivatisation reagent"

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3-Ammino-9-etil carbazolo (AEC) ≥97.0% (by HPLC), LiChropur™ derivatisation reagent, per HPLC, Supelco®

Supplier: Merck

3-Amino-9-ethylcarbazole is a peroxidase substrate suitable for use in immunoblotting and immunohistochemical staining procedures. This substrate produces an insoluble end product that is red in color and can be observed visually.

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HMDS (1,1,1,3,3,3-Esametildisilazano) silylation reagent, for GC derivatisation

Supplier: MACHEREY-NAGEL

HMDS is weak TMS donor. If used as sole reagent, it is slow and not very effective. After addition of catalytic quantities (e.g., 1 %) of TMCS or as a mixture with TMCS it is a fast and quantitative reagent for trimethylsilylation of organic compounds.

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Pentafluorobenzoyl chloride 99%

Supplier: Apollo Scientific

Useful reagent for the derivatisation of primary amines, etc for GC analysis.

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Pentafluorobenzoyl chloride 97%

Supplier: Apollo Scientific

Useful reagent for the derivatisation of primary amines, etc for GC analysis.

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N-Methyl-bis-trifluoroacetamide acylation reagent, for GC derivatisation

Supplier: MACHEREY-NAGEL

This derivitisation reagent is recommended for alcohols, primary and secondary amines as well as for thiols under mild, neutral conditions. MBTFA also forms very volatile derivatives with carbohydrates.

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N,N-Dimethylglycine ≥99% (by total nitrogen and titration analysis), LiChropur™ derivatisation reagent, for HPLC, per LC-MS, Supelco®

Supplier: Merck

N,N-Dimethylglycine is a type of quaternary ammonium compound which exhibits a variety of biological effects.

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N-Methyl-bis-heptafluorobutyramide (MBHFBA) acylation reagent, for GC derivatisation

Supplier: MACHEREY-NAGEL

Acylation with fluorinated bisacylamides is recommended for alcohols, primary and secondary amines as well as for thiols under mild, neutral conditions.

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N-Metil-N-(trimetilsilil)trifluoroacetamide silylation reagent, for GC derivatisation

Supplier: MACHEREY-NAGEL

Silylation in GC stands for replacement of active hydrogen atoms by a trimethylsilyl group (TMS derivative).

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Trimethylsulphonium hydroxide 0.25 M in methanol, Supelco®

Supplier: Merck

Reagent for the methylation of nucleophiles such as carboxylic acids, alcohols, thiols, N-heterocycles. Rapid derivatisation of acids for GC by pre-column transesterification of glycerides.

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Trimethylsulphonium hydroxide 0.2 M in methanol alkylation reagent, for GC derivatisation

Supplier: MACHEREY-NAGEL

Methylation with TMSH is recommended for free acids, chlorophenoxycarboxylic acids, their salts and derivatives as well as for phenols and chlorophenols. Lipids or triglycerides can be converted to the corresponding fatty acid methyl esters (FAMEs) by a simple transesterification.

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N,O-Bis(trimethylsilyl)trifluoroacetamide silylation reagent, for GC derivatisation

Supplier: MACHEREY-NAGEL

BSTFA is a powerful trimethylsilyl donor with approximately the same donor strength as the nonfluorinated analog BSA. Advantage of BSTFA over BSA: greater volatility of its reaction products (particularly useful for GC of some lower boiling TMS amino acids).

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N-(tert-Butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA) silylation reagent, for GC derivatisation

Supplier: MACHEREY-NAGEL

MBDSTFA is a silylation reagent that donates a tert-butyldimethylsilyl group (TBDMS) for derivatizing active hydrogen atoms in hydroxyl, carboxyl and thiol groups, primary and secondary amines as well as amino acids. Fast reactions (typically 5–20 min) with high yields (> 96 %). By-products are neutral and volatile.

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7-Fluorobenzo-2-oxa-13-diazole-4-sulphonic acid ammonium salt ≥98.5% (by HPLC), LiChropur™ derivatisation reagent, per HPLC, Supelco®

Supplier: Merck

Fluorescent probe for thiols and pre-column labeling of biological thiols for HPLC

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2,2,3,3,3-Pentafluoro-1-propanol 98%

Supplier: Apollo Scientific

Suitable reagent for the derivatisation of carboxylic acids for ECD and GCMS analysis.

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N,O-Bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane Silyl-991 silylation reagent, for GC derivatisation

Supplier: MACHEREY-NAGEL

Silyl-991 is recommended for silylation of fatty acid amides, hindered hydroxyls and other compounds which are difficult to silylate (e.g., secondary alcoholes and amines).

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4-Methoxybiphenyl 97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

4-Methoxybiphenyl has been used as a standard reagent whose fluorescence intensity is associated with the fluorescence characteristics of the products of derivatisation reaction for aryl halides with phenylboronic acid (PBA).