buffers

Resistendo alle variazioni di pH, i tamponi proteggono i campioni quando vengono aggiunti acidi o basi. Disponibili in polvere e in forma liquida, tracciano efficacemente le proteine durante l'isolamento e la separazione tramite elettroforesi. I tamponi salati possono anche essere utilizzati per calibrare e standardizzare i pHmetri per ottenere letture accurate. Le sostanze bloccanti impediscono l'adesione degli anticorpi ai siti liberi dalla membrana, che produrrebbero un background interferente. In varie concentrazioni per soddisfare qualsiasi esigenza, i tamponi possono essere disponibili in formato liquido o disidratato.

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Permeabilisation buffer

Supplier: Biotium

Ready-to-use permeabilisation buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

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Buffer, TRIS solution pH 7,5 (1 mol/L)

Supplier: PanReac AppliChem

Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)

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Hybridisation buffers

Supplier: G-Biosciences

The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).

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VWR®, TE (Tris-EDTA) buffer, pH 8,0 for biotechnology

Supplier: VWR Chemicals

Ready to use Tris-EDTA buffer.

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Proteasome assay buffer

Supplier: Enzo Life Sciences

Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.

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Wash buffer concentrate

Supplier: Enzo Life Sciences

Liquid. TRIS buffered saline containing detergents.

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Buffer solutions, ready to use

Supplier: Thermo Fisher Scientific

pH Buffer solution, borato, Valore pH: 9,2, ±0,02, incolore

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Phosphate buffer solution, BioUltra, Supelco®

Supplier: Merck

Phosphate buffer, BioUltra, >0,044 M Na₂HPO₄, >0,022 M NaH₂PO₄, Valore pH: 7,2

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Buffer solution pH 5, Acetate Buffer

Supplier: Thermo Fisher Scientific

Acetate buffer, Contains 0.7 ml glacial acetic acid and 0.6 g potassium hydroxide per litre., Valore pH: 4,98 - 5,02 (20 °C)

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IHC Wash Buffer, Enzo Life Sciences

Supplier: Enzo Life Sciences

The IHC wash buffer is a for washing and removing excess reagents during immunostaining.

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Buffer solutions, according to EP and USP, Certipur®

Supplier: Merck

Buffer solutions intended for extremely precise calibration, qualification and monitoring of pH instruments, and for testing other buffer solutions.

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Gram Negative lysis buffer

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.

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ChIP Buffer, Abcam

Supplier: Abcam

ChIP Buffer for ab185913

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Protein Transfer buffers

Supplier: G-Biosciences

Efficient™ Western Transfer Buffer (20X) achieves increased protein transfer efficiency. This ready-to-use transfer buffer is prepared for optimal conductivity and efficient protein transfer, without generating excessive heat or transfer distortion.

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Wash buffer salts, lyophilised, Enzo Life Sciences

Supplier: Enzo Life Sciences

Wash Buffer Salts can be used in tissue preparations, immunohistochemical staining procedures and in assays that require HRP-linked detection reagents.

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Optimizer Buffer™ for optimal cross-linking and modification

Supplier: G-Biosciences

The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.