buffers

Resistendo alle variazioni di pH, i tamponi proteggono i campioni quando vengono aggiunti acidi o basi. Disponibili in polvere e in forma liquida, tracciano efficacemente le proteine durante l'isolamento e la separazione tramite elettroforesi. I tamponi salati possono anche essere utilizzati per calibrare e standardizzare i pHmetri per ottenere letture accurate. Le sostanze bloccanti impediscono l'adesione degli anticorpi ai siti liberi dalla membrana, che produrrebbero un background interferente. In varie concentrazioni per soddisfare qualsiasi esigenza, i tamponi possono essere disponibili in formato liquido o disidratato.

Permeabilisation, (buffer)

Supplier: Enzo Life Sciences

Permeabilization, (buffer)

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Phosphate Buffer, pH 6.6 at 25 °C

Supplier: SIGMA ALDRICH MICROSCOPY

Phosphate buffer's buffering capacity is pH sensitive and is preferred to be used at physiological pH (7,2 to 7,4).

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Fixation, (buffer)

Supplier: Enzo Life Sciences

Fixation, (buffer)

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VWR®, Buffers, Sodium acetate

Supplier: VWR Chemicals

Sodium acetate buffer B, VWR®

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Buffer, TBE solution 5X concentrate (TRIS-borate-EDTA buffer)

Supplier: Biotium

Running Buffer, TBE buffer solution 5X concentrate (TRIS-borate-EDTA buffer)

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VWR® Ultra Pure Grade, Sm buffer

Supplier: VWR Chemicals

Cloning Buffer, SM BUFFER

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Buffers, concentrated solutions, Titrisol®, Supelco®

Supplier: Merck

Titrisol® buffer concentrate for 500 ml buffer solution [20 °C].

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Buffer solution pH 4, citrate buffer

Supplier: Thermo Fisher Scientific

pH Buffer solution, Citrato, Valore pH: 4,00, ±0,02, incolore

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SIRT assay buffer

Supplier: Enzo Life Sciences

SIRT assay buffer

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Biomol Enzo

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)

Supplier: Thermo Fisher Scientific

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Fixation/Permeabilisation, (buffer)

Supplier: Enzo Life Sciences

Fixation/Permeabilization, (buffer)

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HDAC assay buffer

Supplier: Enzo Life Sciences

HDAC assay buffer

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Electrophoresis running buffers

Supplier: G-Biosciences

There are many electrophoresis running buffers including: TAE running buffer (50×), TBE running buffer (10×), TBE-urea sample buffer, MOPS running buffer (10×), and MOPS/ SDS running buffer (20×). These include the SDS-PAGE sample loading buffer, SDS-PAGE running buffer, native sample loading buffer, tris glycine native gel running buffer, tricine sample buffer, tris-tricine, tris-tricine-SDS, MES SDS running buffer, MOPS/ SDS running buffer, IEF anode buffer, IEF cathode buffer, zymogram sample buffer, zymogram renature buffer, zymogram development buffer and the discontinuous buffer system.