"Staining Solutions"

Supplier: SIGMA ALDRICH MICROSCOPY

Ehrlich’s reagent III, Loeffler’s Methylene Blue. Methylene Blue was earlier recognized as a good nuclear stain, the staining solutions are always either aged or alkalized, if not both. More importantly it is an excellent stain for ribonucleic acid, which is the important stainable constituent of bacterial bodies, of lymphocyte and hemoportoal cytoplasm, and of nerve cell tigroid granules. It can also be employed in capsule stain. The capsule stain begins as a negative stain leaving the organisms colorless. The capsule are clearly seen as halo surrounding the bacterial cell. Another staining procedure is performed with nigrosin (Cat. No. 43925).

Supplier: AAT Bioquest

ProLite™ FAST Blue Protein Gel Stain is a ready-to-use solution.

Supplier: Merck

Ziehl-Neelsen carbolfuchsin solution - for microscopy, is a ready-to use solution used in the Ziehl-Neelsen hot staining for the microscopic investigation of acid-fast bacteria(AFB).

Supplier: SIGMA ALDRICH MICROSCOPY

Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

Supplier: SIGMA ALDRICH MICROSCOPY

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

Supplier: SIGMA ALDRICH MICROSCOPY

Crystal violet solution for use in Brown-Hopps method for Gram-positive and Gram-negative bacterial staining.
Crystal violet solution has been used as a stain in cell proliferation assays, migration assays and Boyden chamber assay.

Supplier: Abcam

Cationic dye. Forms insoluble complexes with glycosaminoglycans in solution. Stains interphase nuclei, especially mitotic figures.

Supplier: SIGMA ALDRICH MICROSCOPY

Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

Supplier: VWR Chemicals

Fixing solution with 0,02% formaldehyde and 0,01% citric acid in aqueous solution.

Supplier: G-Biosciences

The stain uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars. The supplied agent first oxidises the cis-diol sugar groups to aldehydes. The aldehyde groups react with the sensitive glycoprotein stain solution forming Schiff bonds and producing strong magenta colour bands. RAPIDstain™ can be used after glycoprotein staining to detect non-glycosylated proteins and the use of the stain enhances glycoprotein staining. The glycoprotein stainis highly convenient as all the key reagents required for staining are supplied and a unique positive & negative control is included.

Supplier: SIGMA ALDRICH MICROSCOPY

Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.

Supplier: SIGMA ALDRICH MICROSCOPY

Brilliant Blue G is a methanol based stain which is especially designed for staining protein in polyacrylamide gels, but will also stain protein in agarose gels (IEF). The stain contains methanol and acetic acid so gels do not require fixing prior to staining. Water is added directly to the bottle to produce 1 liter of ready-to-use staining solution.

Supplier: Abcam

Abcam's Flow Cytometry Lysing Solution is a ready-to-use solution formulated for lysing erythrocytes following monoclonal antibody staining of whole blood.

Supplier: VWR Chemicals

VWR® Bouin tissue fixative solution.

Supplier: Abcam

Nuclear fast red stain solution (pH 2 - 4) is a stain for histological applications. - Stains nuclei red and cytoplasm pale pink - Contains 80% water, 5% aluminium sulfate, 0.2% nuclear fast red - Enhanced stability - Prevents precipitation over time

Supplier: Biotium

Activated charcoal retains ethidium bromide, GelRed™ and GelGreen™ efficiently in a convenient bag for decontamination of used gel staining solutions. Each bag has a total capacity of at least 10 mg ethidium bromide.