"SIGMA ALDRICH MICROSCOPY"

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Gram’s crystal violet solution, also known as gentian violet or hexamethyl pararosaniline, is a basic, cationic, acidotropic aminoarylmethane dye. It is a hexa-N-methylated triaminotriphenylmethane with a moderately sized lipophilic cation and a slightly nonplanar conjugated system. It is used in bacteriology as the primary stain in Gram staining to differentiate Gram-positive and Gram-negative bacteria. It is known to possess antibacterial and antifungal properties.

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Rhodamine 123 is useful as a laser dye and to have selective cell growth effects. It has also been used to measure mitochondrial membrane potential.

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Dihydrorhodamine 123 has been used for the detection of free intracellular radicals or ROS (reactive oxygen species).

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Biotinylation reagent

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1-Methoxy-5-methylphenazinium methyl sulfate (MPMS) is a photochemically stable electron-transport mediator between NAD(P)H and tetrazolium dyes. The reduced form of MPMS converts tetrazolium salt to purple precipitate formazan. It is a very stable and non-enzymic electron carrier that mediates electron transfer by dehydrogenases in vitro to various electron acceptors such as tetrazolium dyes or the electrode of an enzymic electric cell.

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4-(2-[6-(Dioctylamino)-2-naphthalenyl]ethenyl)-1-(3-sulfopropyl)pyridinium inner salt. ≥95% (HPLC), solid (Di-8-ANEPPS)

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Solvent Blue 38 is a phthalocyanine stain, used for staining myelin, neurons and glial fibres.

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Sudan II has been used to determine its presence in commercial spices such as hot chilli food samples (LC–ESI–MS/MS).

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Acid Blue 80, Dye content 40 %

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Sudan IV is a hydrophobic azo dye. It stains triglycerides and imparts an intense red colour. The dye does not stain lipid droplets or phospholipids. It is also used a diagnostic assay manufacturing, hematology, histology.

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Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. It consists of colloidal silica particles of 15 – 30 nm diameter (23% w/w in water), coated with polyvinylpyrrolidone (PVP).

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Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye. Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.

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2,4,6-Tri(dimetil-aminometile) fenolo, Sigma-Aldrich®

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Crystal Ponceau 6R has been used in the MSB (martius yellow–crystal scarlet–methyl blue) staining procedure for the staining of collagen fibers and erythrocytes.

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This material has a structure similar to Granular Blue (CAS No. 71431-29-3). It has been referred to in some literature as Granular Blue, a fluorescent neuronal tracer.

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Hematoxylin has been used for staining gastric cancer tissues, myocardial tissue, heart sections and ovarian nuclei.