1070 Risultati per: "SIGMA ALDRICH MICROSCOPY"

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D.P.X mounting medium is a mixture of distyrene (polystyrene), a plasticizer (tricresyl phosphate), and xylene. It facilitates the protection of stains and can dry quickly.

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DPX Mountant for histology has been used as a slide mountant to fix sections.

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Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525 nm. It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins. Fluorescein isothiocyanate isomer I has been proposed as a contact sensitiser.

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Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. It is a metachromatic dye suitable for a wide variety of histological staining procedures. A metachromatic dye suitable for a wide variety of histological staining procedures. Toluidine Blue O has been used to stain a variety of specimens like mesenchymal cells, taste buds, osteoblasts, matrix, and more.

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Azure B (azure I) ≥89% (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®

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The polycyclic aromatic hydrocarbon perylene is a hydrophobic fluorescent probe. It is highly photostable and displays blue fluorescence. Perylene is used as a dye for spectroscopic studies.

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Phenazine ethosulfate is a cationic dye and a chemical electron acceptor. It is used in dye-linked enzyme assays. At a high pH, the dye produces free radicals, which may be the actual electron acceptors.

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Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridised to its target.

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Azure A, Sigma-Aldrich®

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(3-Aminopropyl)triethoxysilane forms aminopropyl derivative of glass, an adsorbent for affinity chromatography. It is used to prepare positively charged slides suitable for use with various immunohistochemical and in situ hybridisation procedures.

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Folin & Ciocalteu's phenol reagent is most commonly used in the Lowry method for determining protein concentration. It has also been used for the quantification of total phenolics. In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution
stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750 nm) or shoulder (660 nm) are used to quantitate protein concentrations between 1 - 100 mg/ml while absorbance at 550 nm is used to quantitate higher protein concentrations.

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Bis(cyclohexanone)oxaldihydrazone (Cuprizone) has been used in mice to study the effect of NG2 (neural/glial antigen 2) proteoglycan on toxic-induced demyelination. It has been used to induce demyelination in mice. It has been used to study cuprizone-induced demyelination and remyelination in the corpus callosum of mice.

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Hydroxy naphthol blue disodium salt, ACS reagent.

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Bromophenol Blue–Xylene Cyanol Dye solution has been used in the formamide loading buffer for the size purification of ligated RNA from polyacrylamide gel.

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Phenol Red Sodium Salt (PRSS) is a water-soluble anionic fluorescent dye, that possesses an extra Na ion attached with the SO3 group compared to phenol red. It is intended for use in cell culture applications. A solution of phenol red will have a yellow color at a pH of 6,4 or below and a red color at a pH of 8,2 and above.

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Used for the preparation of electrically conducting glasses. Used to make solutions for the separation of RNA from DNA by density gradient centrifugation.

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For use in the detection of phospholipids and related compounds.

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Bromophenol blue (BroB), an arylmethane color marker dye has a molecular mass of 670 Da.

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Silver enhancer solution A has been used for silver staining.

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Oil red O is a lysochrome (fat-soluble dye) diazo dye used for staining of neutral triglycerides and lipids on frozen sections and some lipoproteins on paraffin sections. In histology, a supersaturated solution of oil red O in isopropanol may be used to stain fat in tissue.

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Designed for orientation of excised surgical specimens. This method of permanently marking the margins of tissue specimens is very beneficial to surgeon and pathologists testing for skin cancer.

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Leukocyte Alkaline Phosphatase (LAP) kits are intended for the semi-quantitative demonstration of alkaline phosphatase activity in white blood cells.

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Cell permeable fluorescent detector of nitric oxide in living cells. 4,5-Diaminofluorescein diacetate (DAF-2 DA) has been used as a fluorescent dye for NO (nitric oxide) detection.

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Rhodamine B is a thiol-reactive and photostable fluorescent dye. Rhodamine B isothiocyanate is an aminoxanthene that possesses carboxylic acid and isothiocyanate substituents on the pendant phenyl ring.

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Prepared by alkaline hydrolysis.

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Gill formulation No. 2 may be used for cytology and/or histology as a progressive or regressive stain depending on length of staining time. 4 g/L certified hematoxylin.

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Carbol Fuchsin is a basic dye which forms a yellow-brown compound in presence of acid. It is mainly required for the staining of mycobacteria. The waxy walls of mycobacteria helps in retaining the dye within cells. It can also be used for the staining of bacterial spores.

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General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.

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Brilliant cresyl blue ALD has been used for the staining of reticulocytes.

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Naphthol AS-D chloroacetate can be used as laboratory chemicals, manufacture of substances.