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Eosina B 1 g/l in soluzione acquosa, Sigma-Aldrich®

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Nuclear counterstain. Included as a component in the Sigma Fetal Hemoglobin Procedure 285.

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Thymoquinone has been used as an inhibitor of Escherichia coli ATP synthase and cell growth. It is also used as a drug against lower limb ischemia-reperfusion injury of sciatic nerve and femoral muscle. Thymoquinone is employed in preclinical studies as a neuroprotective and anticonvulsant agent.

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Methyl Purple,in H2O (Methyl Purple indicator solution)

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Tetrachlorophthalonitrile,98%

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Lugol′s solution is a ready-to-use solution that comprises 6% iodine in 4% potassium iodide. It is commonly used as a mordant for Gram staining in bacteriology. It is used for staining bacterial target structures for diagnostic purposes by fixing, staining, counterstaining, and mounting bacteriological specimen materials.

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Brilliant Blue R has been used in staining of gels after electrophoresis. It has also been used to determine protein in the protein bodies. For detection of protein bands following electrophoresis.

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Used as a kit component for the 91A and 91C procedures for Esterase.

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Kit used to detect neutrophils in peripheral blood, bone marrow or tissue sections which have been paraffin embedded.

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Adrenochrome has been used to study the effect of adrenochrome on coronary artery constriction in the rat heart.

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p-Rosolic acid (C.I. 43800) is an acid-baseindicator from the triphenylmethane group. It is also known as aurin and 4-[Bis(4-hydroxyphenyl)methylene]-2,5-cyclohexadienone.

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Leukocyte Alkaline Phosphatase (LAP) kits are intended for the semi-quantitative demonstration of alkaline phosphatase activity in white blood cells.

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For use with Sigma kit 86R, for the demonstration of alkaline phosphatase in granulocytes. The procedure is often referred to as the LAP procedure.

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o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

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Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.

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Thymol meets analytical specification of Ph. Eur., BP, NF, 99 to 101%.

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Useful for quantitating biotin binding sites. This reagent binds to avidin more quickly than conventional fluorescein biotin.

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Indophenol is used in hair dyes, redox materials, lubricants, liquid crystal displays, biosensor and fuel cells. It is toxic to fishes and is implicated in environmental pollution. Indophenol method is common for the determination of ammonia. The reaction gives a blue product, which is measured spectrophotometrically.

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Used as a kit component in Procedure 91 for the demonstration of specific esterase activity in peripheral blood smears or paraffin sections.

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Sodium 3,5-dichloro-2-hydroxybenzenesulfonate is used in conjunction with 4-aminoantipyrine (4-AAP) and hydrogen peroxide (H2O2) for chromogenic quantitation of uric acid in serum and urine samples.

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Used to demonstrate polymorphonuclear cells in blood or bone marrow films. Each kit has sufficient reagents for 10 uses.

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4-(2-Pyridylazo)resorcinol monosodium salt is a chromophoric indicator and chromogenic chelator employed for the spectrophotometric determination of several metal ions.

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Intended for use in Sigma Procedure 387 for the demonstration of Acid Phosphatase (TRAP).

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Azure B tetrafluoroborate, Dye content 95 %

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New Coccine has been used to determine its decolorization by fungi Phanerochaete chrysosporium and Pleurotus sajorcaju.

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Naftolo AS-MX fosfato, Sigma-Aldrich®

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When blood films are stained using Wright-Giemsa Stain, the white blood cell nucleus and cytoplasm take on the characteristic blue or pink coloration. The combination of purified eosin and thiazine dyes in the product eliminates inconsistent staining and yields reproducible chromogenic responses.

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Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

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Fluoroshield™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

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A fluorescent conjugate of bisindolylmaleimide that specifically binds phosphokinase C at the active site. Can monitor enzyme activation and translocation. This reagent is not membrane permeable. The diacetate form (Sigma No. F9801) is membrane permeable.