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Mounting Medium, CC/Mount™

Supplier: SIGMA ALDRICH MICROSCOPY

CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.

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Histopaque®, sterile filtrato, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

ACCUSPIN System-Histopaque-1077 products are intended for use in the isolation of lymphocytes and other mononuclear cells. The separation medium, Histopaque-1077, is a sterile-filtered, endotoxin-tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1,077 g/ml. ACCUSPIN System-HISTOPAQUE-1077 employs γ-irradiated 50 mL polypropylene centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ('frit'). Each tube is aseptically filled with 15 ml of Histopaque 1077. Each tube will separate 15 to 30 ml of anticoagulated blood.

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Novec™ 7100 engineered fluid

Supplier: SIGMA ALDRICH MICROSCOPY

Novec™ 7100 fluid consists of two inseparable isomers ((CF₃)₂CFCF₂OCH₃) and CF₃CF₂CF₂CF₂OCH₃) with essentially identical properties. Ideal for vapor degreasing applications as a neat (pure), azeotropic component and co-solvent parts cleaner.

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Alcian Blu soluzione, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.

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Zinquin ethyl ester ≥95% (by HPLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Zinquin ethyl ester. ≥95% (HPLC), solid (2-Methyl-8-[(4-methylphenyl)sulfonylamino]-6-(ethyloxycarbonylmethyloxy)quinoline)

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Wright's stain soluzione, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Wright stain solution was used during the exudate analysis of white blood cell concentration.

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Auramina O 85% (dye content) certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta. Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall. Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

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Soluzione Iodio di Lugol per microscopia, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Lugol solution, for microscopy (Bact., Bot.) (Iodine/Potassium iodide solution).

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Naftoresorcina ≥99.00%, crystalline, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

1,3-Dihydroxynaphthalene is a dye intermediate forming stable colors when applied with particular mordants and in mixtures with other oxidising dyes. This specific reagent is intended for the quantitative colorimetric determination of alduronic acids, such as glucuronic acid from urine. The mutagenicity of ozonation derivatives of 1,3-dihydroxynaphthalene against several Salmonella typhimurium strains has been studied.

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Cristal Violetto, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Mixture of crystal violet [C.I.No. 42555] and methyl violet [C.I.No. 42535]. Dye employed for preparing Gram stain.

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Azo rubine (50% dye content) C.I 14720 , Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Chromotrope FB, also known as acid red 14 (AR14), is a bright orange-red powder. It belongs to the class of monoazides.

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Pyridine-3,4-dicarbonitrile, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Pyridine-3,4-dicarbonitrile, Sigma-Aldrich®

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DiBAC₄(3), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Application in high throughput screening.

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4',6–Diamidino-2-fenilindolo dilactato (DAPI) ≥98% (by HPLC) used in nuclear staining, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

A high sensitivity dye used to detect single nucleic acid molecules. DAPI has been shown to detect a single DNA molecule in solution, and to detect femtograms of DNA in single cells and chloroplasts.

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Brilliant black BN, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Brilliant Black BN has been used: in micronucleus and comet assays to evaluate its genotoxic effect.as a quenching agent in calcium mobilization assay.

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Direct Blue 71 50% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Direct Blue 71, Dye content 50 %

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Acido 3,5-dinitrosalicilico, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acido 3,5-dinitrosalicilico, Sigma-Aldrich®

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Cresol red sodium salt, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Cresol red sodium salt, Sigma-Aldrich®

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Erioglaucine disodium salt, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Erioglaucine disodium salt has been used as a coloring dye to determine the feeding behaviour in Drosophila. It has been used as a coloring dye for determining the number of egg masses of Meloidogyne incognita.

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Turkey red oil sodium salt per microscopia, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Turkey red oil sodium salt per microscopia, Sigma-Aldrich®

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1,3-Diiminoisoindoline ≥97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

1,3-Diiminoisoindoline,97% (1,3-Isoindolinediimine; Phthalimide diimide)

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Lucifer Yellow VS dilithium salt ~85%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Lucifer Yellow VS dilithium salt ~85%, Sigma-Aldrich®

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Renin Substrate 1, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Substrate for human renin, an aspartic protease which plays an important role in blood-pressure regulation.

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(2,5-Dimethoxyphenyl)acetyl chloride 99%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

(2,5-Dimethoxyphenyl)acetyl chloride 99%, Sigma-Aldrich®

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2,6-Dichloroquinone-4-chloroimide, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

2,6-Dichloroquinone-4-chloroimide, Sigma-Aldrich®

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Methyl-3-amino-5,6-dichloropyrazine-2-carboxylate 97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Methyl-3-amino-5,6-dichloropyrazine-2-carboxylate 97%, Sigma-Aldrich®

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Buffer concentrate, citrate phosphate, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

These buffers are used to elute adult hemoglobin by the procedure of Oski and Naiman, a modification of the Kleihauer Betke procedure for the demonstration of fetal hemoglobin.

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Rhodanile blue 75% (dye content), solid, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Rhodanile blue 75% (dye content), solid, Sigma-Aldrich®

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MBD

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescent probe for hydrophobic (non-polar) regions in proteins and nucleoproteins.

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Naphthol AS-BI phosphate soluzione 12,5 mg/mL in DMF, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Naphthol AS-BI Phosphoric acid solution foruse in Sigma Acid Phosphatase Procedure No. 386.

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