1070 Risultati per: "SIGMA ALDRICH MICROSCOPY"

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Fura 2-AM (Fura 2) is a calcium-specific ratio-metric fluorescent dye. It is also known as acetoxy-methyl-ester. In response to calcium ions, ratio metric dyes like Fura 2 changes their respective excitation or emission spectra. The intracellular calcium concentration is determined by the fluorescence emission ratio or excitation at distinct wavelengths. Fura 2 has an emission peak at 505 nm, and the excitation peak ranges between 340 to 380 nm and is the preferred calcium indicator.

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5-Fluorouracil has been used to induce apoptosis in cells. It has been used as a chemosensitising agent. A potent antitumor agent that affects pyrimidine synthesis by inhibiting thymidylate synthetase, thus depleting intracellular dTTP pools. It is metabolized to ribonucleotides and deoxyribonucleotides, which can be incorporated into RNA and DNA. Treatment of cells with 5-FU leads to an accumulation of cells in S-phase and has been shown to induce p53 dependent apoptosis.

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The release of phosphate or pyrophosphate in an enzymatic reaction can be detected by malachite green and is measured spectrometrically at 660 nm.

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Blu metilene ≥82% (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®

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Eosina-blu di metilene sec. May-Grünwald soluzione, Sigma-Aldrich®

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Eosina-blu di metilene sec. May-Grünwald soluzione, Sigma-Aldrich®

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Phenol Red is a phenolsulfonphthalein compound. It is present as a sulfonated hydroxyquinone in weakly acidic aqueous solutions. In alkaline solutions, the dye forms a hydrophilic dianion (a small, red anion and has a larger conjugated system than the yellow hydroxyquinone).

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Phloxine B has been used as a component of yeast extract peptone dextrose (YEPD) to grow Schizosaccharomyces pombe.

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This is the pure, principal component of mixtures commonly referred to as Basic Fuchsin.

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Jenner's stain,certified by the Biological Stain Commission (Jenner Stain; Eosin-Methylene Blue)

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Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.

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Blu di Toluidina O, Sigma-Aldrich®

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Disperse blue 1 is a grey to black powder. Disperse dyes are known to be insoluble in water and are applied to the substrate as suspensions or dispersions by dissolving in hydrophobic regions of the polymers making up the fiber.

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New Methylene Blue N zinc chloride double salt has been used as a photosensitizer for the in vitro photodynamic inactivation of plant-pathogenic fungi Colletotrichum acutatum and Colletotrichum gloeosporioides. It has also been used to determine the in vitro susceptibilities of Trichophyton mentagrophytes and Trichophyton rubrum microconidia to photodynamic antimicrobial chemotherapy (PACT).

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Gram’s safranin solution, also known as Safranin T or Basic Red 2, is a ready-to-use staining solution that comprises 10% safranin in ethanol. Safranin is a basic, cationic, azine dye containing a planar, diaminophenazine chromophore, with a phenyl substituent attached to a ring nitrogen. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. It is most notably used in bacteriology as a counterstain in Gram staining to differentiate between Gram-positive and Gram-negative bacteria.

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N,N-Dimethyl-4,4′-azodianiline, also knows as Disperse Black 3 is a disperse azo dye. It has been used in a condensation reaction for the systhesis of azo-based phenylthiophene Schiff bases.

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N,N-Dibutylaniline 97%, Sigma-Aldrich®

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Methyl red hydrochloride in ACS Reagent grade water, Sigma-Aldrich®

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Sunset yellow FCF is a food dye, which is is substantially used in various food preparations. It is a orange-red coloured crystal, that is soluble in water and ethanol. It is used in treating bone metabolic diseases, dermatological disorders, respiratory illness etc. It can alter the functional responses of splenocytes at non-cytotoxic dose. It promotes immune suppression. Relative expression of surface receptors in T and B cells can be altered by sunset yellow FCF.

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Acid Blue 113, Dye content 50 %

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Formvar soluzione ~1% in 1,2-dichloroethane per microscopia, Sigma-Aldrich®

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Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

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Disperse Orange 37 may be used during an experimental study conducted to understand the mutagenic and carcinogenic potential of textile azo dye processing plant effluent, that is known to have an impact on drinking water source.

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4-Amino-3,6-disulfo-1,8-naphthalic anhydride dipotassium salt has been used to synthesise the fluorescent peptide analog, Lucifer yellow anhydride.

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Neutral red (C.l. 50040, Cl Basic red 5) is a protonated diaminophenazine dye that has a small planar conjugated system.

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An important fluorophore prepared in high purtity with a detection limit of TMR-labeled amino acids by capillary electrophoresis of approx. 600 molecules. Readily excited by the 546 nm spectral line from mercury-arc lamps used in fluorescent microscopes, and is intrinsically more photostable than fluorescein.

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2,3-Diaminonaphthalene is also known as 2,3-Naphthalenediamine or DAN. It is one of the isomers of naphthalene bearing two amino groups, which are also called naphthalenediamines. They are usually prepared by reducing the nitronaphthalene derivative and aminating the subsequent hydroxynaphthalene derivative.

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2-(3-Aminophenylsulphonyl)ethanol hydrochloride 97%, Sigma-Aldrich®

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Standard stain for microscopy. It may be also be used as a reference standard in the determination of azocarmine G in food products and beverages using high performance liquid chromatography coupled with diode array detector (HPLC-DAD).

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N,N'-Ditridecyl-3,4,9,10-perylenetetracarboxylic diimide 95%, Sigma-Aldrich®