1070 Risultati per: "SIGMA ALDRICH MICROSCOPY"

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4'-Hydroxybiphenyl-4-carboxylic acid 99%, Sigma-Aldrich®

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Phenosafranin has been used for nuclear staining.

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4-(2-(6-(Dibutylamino)-2-naphthalenyl)ethenyl)-1-(3-sulfopropyl)pyridinium hydroxide inner salt has been used as a voltage sensitive dye in cardiac cells.

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Tetramethylrhodamine methyl ester perchlorate (TMRM) is a fluorescent dye that can label mitochondria and mark embryonic stem cell/induced pluripotent stem cell-derived cardiomyocytes. It is useful for the isolation of mature cardiomyocytes.

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Formaldehyde is both a non-coagulant and an additive fixative. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining. Although there is no true universal fixative, 10% neutral buffered formalin (NBF) is perhaps the most commonly used fixative throughout the world for light microscopy and is a somewhat forgiving fixative. Of the dozens and dozens of available fixatives, it is hard to imagine a fixative more commonly used than 10% NBF. 10% neutral buffered formalin is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue.

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7-Amino-4-(trifluoromethyl)coumarin (AFC) is a novel fluorescent marker used for the detection of proteinases. Suitable as laser dye.

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Alcian Blue 8GX is used as a heteroglycan stain for neutral, sulfated and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices.

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Intended for use in Sigma Gram Stain Procedure, HT90.

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Novec™ 7100 fluid consists of two inseparable isomers ((CF₃)₂CFCF₂OCH₃) and CF₃CF₂CF₂CF₂OCH₃) with essentially identical properties. Ideal for vapor degreasing applications as a neat (pure), azeotropic component and co-solvent parts cleaner.

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Coomassie Violet R200, Dye content 50 % (Acid violet 17)

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Histopaque®-1119 is a sterile, endotoxin tested, ready-to-use medium. This solution consists of polysucrose and sodium diatrizoate, adjusted to a density of 1,119 g/ml and pH 8,8 to 9,0.

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Building block for 'Anti-Bredt' strained molecules.

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ANSA (8-Anilino-1-naphthalenesulfonic acid) is a hydrophobic polarity sensitive fluorescent dye useful as a site probe to detect conformational changes in cell and micelle membranes and molecules such as proteins. ANSA is also used as a fluorescent probe for the estimation of the critical micelle concentration (CMC) of surfactants.

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Ethyl 3,5-dimethyl-2-pyrrolecarboxylate,98%

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For use in the Naphthol AS-D Chloroacetate Esterase and Naphthyl Acetate Esterase Procedures, Kits 91C and 91A.

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Kit component in the Masson Trichrome procedure, catalog number HT15

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Ehrlich’s reagent III, Loeffler’s Methylene Blue. Methylene Blue was earlier recognized as a good nuclear stain, the staining solutions are always either aged or alkalized, if not both. More importantly it is an excellent stain for ribonucleic acid, which is the important stainable constituent of bacterial bodies, of lymphocyte and hemoportoal cytoplasm, and of nerve cell tigroid granules. It can also be employed in capsule stain. The capsule stain begins as a negative stain leaving the organisms colorless. The capsule are clearly seen as halo surrounding the bacterial cell. Another staining procedure is performed with nigrosin (Cat. No. 43925).

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3-(2-Pyridyl)-5,6-di(2-furyl)-1,2,4-triazine-5′,5′′-disulfonic acid disodium salt, also known as Ferene, is commonly used to measure the concentration of iron ions in aqueous solutions. The reagent is sensitive to ferrous iron ions (Fe2+) and forms a purple-colored complex with these ions in the presence of a reducing agent. The intensity of the purple color formed is proportional to the concentration of iron ions present in a solution and is quantified spectrophotometrically.

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4-(2-Pyridylazo)resorcinol (PAR) is a monosodium salt(1), dibasic acid that forms the protonated complexes with most metal ions.

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It is used for the preparation of electrically conducting glasses. Used to make solutions for the separation of RNA from DNA by density gradient centrifugation.

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Toluidine blue (C.l. 52040) is a diaminothiazine dye. It is also known as TBO, tolonium chloride, and toluidine blue O.

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Rhodamine 110 chloride (R110) is a laser grade dye. It has been used for the synthesis of the rhodamine 110 octadecyl ester (R110C18).

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3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridisation. DAB (3,3′-Diaminobenzidine) is utilised in many applications for the visualisation of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidised DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.

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Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.

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Methylene blue, Neisser formulation. Well developed granules of volutin (polyphosphate) may be seen in unstained wet preparations as round refractive bodies within the bacterial cytoplasm. With methylene blue they stain metachromatically, a reddish purple color.

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Chicago Sky Blue 6B is a counterstain for background autofluorescence in fluorescence and immunofluorescence histochemistry. It has also been used as a filling in glass pipette electrodes for electrophysiological recordings in the brain sections of rats.

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10-(2',4'-Dinitrophenylazo)-9-phenanthrol, (DNAF)

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Phycobiliprotein exhibiting exceptional absorbance and fluorescence characteristics due to a lack of susceptibility to internal and external fluorescence quenching. Ideal for highly sensitive studies in flow cytometry and immunoassays.

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Suitable for titration of morpholine

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Verde brillante (idrogenato di zolfo) ≥90% (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®