"Quantabio"

Supplier: Quantabio

Faster, better, tougher next generation cDNA SuperMix for cDNA synthesis.

Supplier: Quantabio

PERFECTA® qPCR ToughMix® è un 2X concentrato pronto per l'uso di cocktail di reazione per l'amplificazione PCR di modelli di DNA che supera molti noti inibitori del PCR, spesso presenti nei campioni di greggio estratto da campioni ambientali, tessuti vegetali, o tessuti animali. E 'un Real-Time qPCR reagente versatile e robusta che garantisce la massima sensibilità ed efficienza PCR con una varietà di sonde chimiche fluorogeniche, tra cui le sonde di idrolisi TaqMan®. PERFECTA® qPCR ToughMix® contiene tutti i componenti necessari di reazione, ad eccezione di primer, sonde (s) e il modello di DNA. Il colore azzurro del colorante tracciante AccuVue ™ semplifica il montaggio reazione in bianco piatti, o chiari e aiuta a minimizzare pipettando o errori di miscelazione. Non interferisce con le prestazioni qPCR o influenzare la stabilità del prodotto.

Supplier: Quantabio

AccuStart™ II GelTrack PCR SuperMix is a 2X concentrated, ready-to-use reaction cocktail for routine PCR amplification of DNA fragments up to 4 kb followed by analysis on agarose gels. It contains all components, except primers and template. AccuStart™ II GelTrack PCR SuperMix simplifies reaction assembly, improves assay reproducibility, and reduces the risk of contamination. The supermix includes electrophoresis tracking dyes that migrate at approximately 4 kb and 50 bp to allow direct loading of PCR product on agarose gels following amplification. AccuStart™ II Taq DNA polymerase in the master mix is inactivated with monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly.

Supplier: Quantabio

sparQ PureMag beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads; this product can be used to quickly remove primers, primer-dimers, unincorporated nucleotides, salts, adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing, allowing seamless integration into existing workflows.

Supplier: Quantabio

Le miscele PerfeCTa® qPCR SuperMixes con concentrazione 2X sono assortimenti di reazioni pronte all'uso e contengono tutti i componenti necessari eccetto i primer, la sonda e il templato per qPCR.

Supplier: Quantabio

AccuStart™ II Taq DNA Polymerase is a high purity, recombinant Taq DNA polymerase preparation with high avidity monoclonal antibodies that bind the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation (1 minute at 94 °C), the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Non-specific extension of primers at low temperatures is a common cause of artifacts and poor sensitivity in PCR. The AccuStart™ II automatic hot-start enables specific and efficient primer extension in the PCR process with the added convenience of room temperature reaction assembly. The included 10X PCR Buffer II is a new optimised buffer that provides higher product yield, improved specificity, and enhanced multiplexing capability.

Supplier: Quantabio

An optimised kit for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms and prepares for sequencing success with the highest quality library.

Supplier: Quantabio

qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ Taq DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.

Supplier: Quantabio

Superior sensitivity and multiplexing for DNA amplification of long targets.

Supplier: Quantabio

PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready to use reaction cocktail for unbiased, selected enrichment of target sequences from limited amounts of starting material for downstream gene expression profiling or targeted resequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualise small reaction volumes and ensure accurate pipetting.

Supplier: Quantabio

PerfeCTa® qPCR FastMix® II is an advanced qPCR reagent system for both fast and conventional PCR cycling protocols or instruments. It is a versatile and robust solution that provides the ultimate sensitivity and high PCR efficiency using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR FastMix® II is provided as a 2X concentrated ready to use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

Supplier: Quantabio

Questi kit offrono una soluzione pratica ed estremamente sensibile per RT-PCR in Real Time (qRT-PCR) di templati a RNA mediante l'uso di sonde d'ibridazione come le Taqman® a idrolisi al 5'.

Supplier: Quantabio

sparQ UDI Adapters (1-96) are unique dual-indexed adapters designed to be used on illumina sequencing platforms. It is compatible with both DNA and RNA libraries. The use of UDI’s minimises index hopping events while also enabling greater multiplexing on flow cells.

Supplier: Quantabio

FastMixes include tamponi e stabilizzatori ottimizzati per la massima efficienza, riproducibilità e sensibilità della PCR con sonde TaqMan® e TaqMan® MGB. Una componente chiave delle miscele PerfeCTa® qPCR FastMixes è la Taq DNA polimerasi AccuFast™ che consente un'attivazione istantanea e una cinetica per una polimerizzazione rapida. Durante l'attivazione termica (20 secondi a 95°C), gli anticorpi denaturano irreversibilmente rilasciando la Taq DNA polimerasi completamente attiva e non modificata. Ciò promuove l'estensione specifica ed efficace del primer con la comodità di una fase di preparazione della reazione a temperatura ambiente. Le miscele FastMixes assicurano massima efficienza, sensibilità e specificità della PCR e un robusto segnale fluorescente con reazioni PCR standard o rapide.

Supplier: Quantabio

Ultra-sensitive mRNA library prep workflow with efficient poly(A) capture.

Supplier: Quantabio

AccuStart™ Genotyping ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes.