"PCR Mastermixes"

Supplier: Quantabio

La miscela SuperMix con concentrazione 2X è un assortimento di reazioni pronte all'uso e contiene tutti i componenti necessari eccetto i primer, le sonde e i templati. Questo sistema consente di superare i molteplici limiti delle miscele Master per PCR tradizionali, consentendo l'amplificazione non affetta da errore sistematico di fino a cinque sequenze target in un'unica provetta. L'occultamento di ampliconi a basso numero di copie da parte di target di riferimento con numero di copie più elevato durante l'amplificazione è un problema comune nella PCR multiplex. Ciò può deviare o mascherare la rappresentazione e la quantificazione delle sequenze target a basso numero di copie. PerfeCTa® MultiPlex qPCR SuperMix offre una' elevata sensibilità paragonabile a quelle dei saggi mediante sonda in qPCR singleplex senza dover limitare o variare le concentrazioni dei primer.

Supplier: Q BIOANALYTIC

Vibrio alginolyticus causes eye, ear and wound infections. It can be tested using this Real-Time PCR test. The analysis can be done in water and food samples. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

The Real-Time PCR screening kit is addressing three different targets: 35S promoter; NOS Terminator; CTP2-CP4 EPSPS. The tests are all in accordance with the JRC guideline. Advantage of the CTP2-CP4 EPSPS compared to FMV detection is that the used target includes artificial sequences, which are not present naturally like the virus sequence of FMV.

Supplier: Quantabio

La sintesi di cDNA e l'amplificazione PCR vengono eseguite nella stessa provetta senza necessità di continue aperture. Questi kit sono ottimizzati per consentire massima efficienza e specificità della RT-PCR e consentono coamplificazione non affetta da errore sistematico nelle trascrizioni di RNA target a basso numero di copie in presenza di geni di riferimento presenti con numero di copie più elevato. Pertanto, i kit qScript™ One-Step qRT-PCR offrono una migliore sensibilità rispetto agli altri reagenti qPCR reperibili in commercio. La miscela qScript™ One-Step Master Mix massimizza le attività sia della trascrittasi inversa che degli enzimi della PCR, riducendo al minimo la formazione di dimeri di primer. L'alta specificità è assicurata dalla Taq DNA polimerasi AccuStart™ legata da anticorpi monoclonali. Al raggiungimento della temperatura di 95 °C, gli anticorpi denaturano irreversibilmente rilasciando la Taq DNA polimerasi completamente attiva e non modificata. I kit qScript™ One-Step qRT-PCR sono disponibili per tutti gli strumenti per PCR Real time, comprese quelle che richiedono la normalizzazione mediante colorante di riferimento ROX.

Supplier: Q BIOANALYTIC

Testing for pathogenic E. coli like EHEC, EPEC , EIEC etc. can help to prevent foodborne infections as it has taken place in 2011 with E. coli strain O104 in Germany and with strain O157 at other places in the world. The real-time PCR kit allows the detection of stx1, stx2 and the eaeA gene which covers all different strains of pathogenic E. coli. The test can also be used for the confirmation of bacterial colonies.

Supplier: Q BIOANALYTIC

OneCup it‘s so easy! The OneCup assay comes along with a minimum of pipetting steps. Everything is included in the mix. You only need to combine 20 μl of the mix with 2 μl of your DNA sample and everything is ready. You have a minimum of hands-on time and avoid mistakes during pipetting. You can run the test on all common block cyclers. Application: Detection of Salmonella in food samples and environmental samples subsequent to a pre-enrichment step according to DIN EN ISO 20837 and 20838. In combination with an appropriate pre-enrichment and DNA preparation the test complies with the official method of the German food and feed law §64. ASU L00.00-98. Test principle: The test is dedicated to detect DNA of Salmonella in a sample using Real-Time PCR in compliance with the ISO method mentioned below. The kit uses the TaqMan® principle. Thus the requirements of the ISO standard, that an amplification product has to be confirmed by a hybridization step, is fulfilled. Each reactions contains an internal amplification control. Therefore, false negative results due to inhibition of the reaction can be excluded. The test included the UNG enzyme to prevent re-amplification of contaminating amplicons.

Supplier: Q BIOANALYTIC

Vibrio vulnificus causes severe wound infections and is harmful when ingested. 33% of patients being infected die. The detection in food and water samples using this Real-Time PCR Test is very rapid and versatile. You have results available already after 24 hours. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Legionella spec. is tested usually with cultural methods. However, this may take up to 14 days to deliver results and some strains are living but are not culturable. Using this Real-Time PCR test you may have results already after a few hours. You can use this test in combination with an appropriate pre- enrichment or you can do a DNA purification directly from the filter without pre-enrichment. The kit is also suitable to confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Testing for pathogenic E. coli like EHEC, EPEC , EIEC etc. can help to prevent foodborne infections as it has taken place in 2011 with E. coli strain O104 in Germany and with strain O157 at other places in the world. The real-time PCR kit allows the detection of stx1, stx2 and the eaeA gene which covers all different strains of pathogenic E. coli. It also includes a test for Shigella. The test can also be used for the confirmation of bacterial colonies.

Supplier: Q BIOANALYTIC

Kit for Vibrio cholerae tox gene (ctx). Food safety experts like the German DGHM recommend to test warm water fish for vibrio cholerae. With this Real-Time PCR test you have a very versatile method for testing whether or not the present strain has the ability to produce toxins. You can use our Real-Time PCR Test for Vibrio cholerae for testing for the presence of the species before. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Q BIOANALYTIC

Vibrio parahaemolyticus tox gene (tdh/trh): Food safety experts like the German DGHM recommend to test warm water fish for Vibrio parahaemolyticus. With this Real-Time PCR test you have a very versatile method for testing whether or not the present strain has the ability to produce toxins (tdh/trh). You can use our Real-Time PCR Test for Vibrio parahaemolyticus for testing for the presence of the species before. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Quantabio

Ultra-Plex™ 1-Step ToughMix® is a ready-to-use, 4X-concentrated master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridisation probe detection chemistries such as TaqMan® 5’-hydrolysis probes on Real-Time PCR systems that utilise ROX passive reference with 490 nM excitation. First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets in uni- or multiplexed RT-qPCR applications as well as high throughput gene-expression studies. The system has been optimised to deliver maximum RT-qPCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times. Ultra-Plex™ 1-Step ToughMix® contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries.

Supplier: Q BIOANALYTIC

Food safety experts like the German DGHM recommend to test warm water fish for vibrio cholerae . With this Real-Time PCR test you have a very versatile method for food testing, which can gives you results within 24 hrs. The test was developed and validated within the German Network for Vibrio Research 'VibrioNet' with many scientific institutions involved. It can be applied for food and sea water samples. In case of positive results you may be interested to determine whether or not the present strain has the ability to produce toxins. You can use our Real-Time PCR Test for toxicity gene of Vibrio cholera for this purpose VWR catalogue number: QBIAQB-RTI-19. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: MP Biomedicals

Taq-&GO™ is a ready to use optimised reaction mix for PCR.

Supplier: Q BIOANALYTIC

Food safety experts like the German DGHM recommend to test warm water fish for Vibrio parahaemolyticus. With this Real-Time PCR test you have a very versatile method for food testing, which can gives you results within 24 hrs. The test was developed and validated within the German Network for Vibrio Research “VibrioNet” with many scientific institutions involved. It can be applied for food and sea water samples. In case of positive results you may be interested to determine whether or not the present strain has the ability to produce toxins (tdh/trh). You can use our Real-Time PCR Test for toxicity gene of Vibrio cholera for this purpose VWR catalogue number: QBIAQB-RTI-50. You can use this test in combination with an appropriate pre- enrichment in alkaline peptone-water or you can confirm bacterial colonies on agar plates.

Supplier: Thermo Fisher Scientific

Il sistema qRT-PCR Verso™ combina l'enzima RT Verso™ e il Master Mix ABsolute™ Blue qPCR per garantire un prodotto estremamente economico e versatile. Disponibile in formato mono o bifase, in confezioni multiple, per adattarsi ai requisiti di ogni applicazione e resa.