"G-Biosciences"

Supplier: G-Biosciences

T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes. The protein forms a right-handed nucleoprotein complex on single ssDNA called the presynaptic filament that can search for homology in duplex DNA and pair the recombining DNA molecules to form a DNA joint molecule. This process is aided by ssDNA binding proteins and recombination mediators. The filament is then elongated by DNA polymerase with the newly synthesised strand displacing the old strand. The newly synthesised duplex DNA can then act as a template for the next cycle to achieve exponential amplification of dsDNA. E.coli cells expressing recombinant T4 UvsX gene.

Supplier: G-Biosciences

Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues and typically there is little or no cleavage at arginyl-proline and lysyl-proline bonds. The distribution of these residues in proteins allows trypsin digestion to produce peptides that are readily identified by mass spectrometry.

Supplier: G-Biosciences

Recombinant human caspases 1 - 3 and 6 - 10 are supplied lyophilised in quantities of 25 units. One unit of the specific recombinant caspase is the enzyme activity that cleaves one nanomole of a specific caspase substrate per hour at 37 °C in a reaction solution containing 50mM HEPES (at a pH of 7,2), 50 mM NaCl, 0,1% CHAPS, 10 mM EDTA, 5% glycerol and 10 mM DTT.

Supplier: G-Biosciences

CDI amine reactive agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.

Supplier: G-Biosciences

Chromogenic substrate for alpha-D-glucosidase yielding a yellow solution upon cleavage. Also used for the detection of glucansucrases and for yeast alpha-D-glucosidase and for studies of the crystal structure of the complexes of concanavalin A.

Supplier: G-Biosciences

HOOK™ IgG biotinylation kits are designed for the biotinylation of IgG molecules by immobilising the IgG molecules on a solid support. Nickel chelating resin allows for the rapid removal of uncoupled biotin and therefore eliminates the need for further dialysis or desalting of the biotinylated antibody.

Supplier: G-Biosciences

CytoScan™ LDH Cytotoxicity assay quantitatively measures the cytosolic, lactate dehydrogenase (LDH) enzyme, which is released from damaged cells. The released LDH is measured with a coupled enzymatic reaction that results in the conversion of a tetrazolium salt (iodonitrotetrazolium; (INT)) into a red color formazan by diaphorase. The resulting formazan is measured at 490 nm.

Supplier: G-Biosciences

FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.

Supplier: G-Biosciences

QuenchShield™ Mountant is glycerol based aqueous mountant, which prevents quenching of fluorescence of fluorescent labeled cells and tissues samples during fluorescence microscopy experiments. In addition, it helps preserve the fluorescence of stored slides.

Supplier: G-Biosciences

The Reversible Copper Stain™ is a single step stain for the rapid detection of proteins resolved on SDS-PAGE.

Supplier: G-Biosciences

RUBEO™ is a fluorescent protein stain that is ideal for staining proteins in 1D and 2D electrophoresis gels.

Supplier: G-Biosciences

A chromogenic substrate widely used for detecting sulfatase enzyme activity. Upon enzymatic hydrolysis by arylsulfatase, it releases 5-bromo-4-chloro-3-indoxyl, which oxidizes to form a blue-green insoluble dye. This property makes it particularly useful for histochemical staining, enzyme localisation, and diagnostic assays, especially in microbiological and biochemical research.

Supplier: G-Biosciences

FISH-Blocker™ uses a fish protein as the primary blocking agent to eliminate cross reactivity. Using a non mammalian protein eliminates or minimises the interaction of antibodies raised in mammals. It is offered either in TBS (Tris-buffered saline) or in PBS (phosphate-buffered saline) at pH 7,5.

Supplier: G-Biosciences

Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.

Supplier: G-Biosciences

LabSafe™ Nucleic Acid Stain is a safe nucleic acid stain for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels.

Supplier: G-Biosciences

Chromogenic beta-galactosidase substrate yielding a yellow solution upon cleavage. Can be used for the detection of coli forms due to their beta-galactosidase activity.