"EDVOTEK"

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Four experimental modules are combined into one experiment to provide a comprehensive biotechnology exploration focusing on the green fluorescent protein (GFP). Bacterial cells are transformed to express the GFP. The transformed cells are then grown and the GFP purified by column chromatography. Finally, the purity of the protein fractions are analysed by SDS polyacrylamide electrophoresis.

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According to the World Health Organisation, between 2 and 3 million cases of skin cancer occur globally every year. Many of these cases are caused by preventable damage to DNA by UV light. In this experiment, students expose plasmid DNA to shortwave UV light to simulate the effect of sunbathing. The DNA is then analysed by agarose gel electrophoresis to observe the damage.

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Cancer cells differ from normal cells by the combinations of proteins that are present on their surfaces. Antibodies against these proteins will specifically bind to cancer cells and not to normal cells. This allows early detection of cancer and potentially a way of delivering cancer therapies. In this simulation experiment the reaction of cancer cell markers and their corresponding antigens are demonstrated.

Supplier: EDVOTEK

Oil spills cause devastation to the environment, killing sea life, birds, and coastal plants. Spraying areas of contamination with oil-eating microbes accelerates the degradation of the oil. This process is known as bioremediation. In this open-ended experiment, students grow a mixture of oil-eating bacteria and observe their effectiveness at degrading a variety of oils.

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When normal cells are grown in culture they stop growing when they become overcrowded (contact inhibition). Cancer cells in culture grow in an uncontrolled way because they have lost this property. This helps tumours to form in the body. In addition, many different cell types can be present in a single tumour. This experiment allows students to see the differences between normal and cancer cells in both their growth and cell types.

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The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used extensively in all areas of science. Many organisms have been transformed with the GFP gene, the gene responsible for bioluminescence in jellyfish. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment, students transform bacteria either by GFP, BFP or both.

Supplier: EDVOTEK

This experiment uses PCR to amplify a small section of Lambda DNA via a 2-step process, saving valuable classroom time and allowing completion of the lab in one session.

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FlashBlue™ is a DNA stain that offers simple and rapid staining of agarose gels.

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Southern blotting is an important technique used widely in clinical genetics and research. By transferring DNA from an agarose gel onto a membrane the method allows the precise analysis and identification of DNA bands on a gel. Using this kit, students use Southern blotting to find a point mutation in the haemoglobin gene indicating Sickle Cell Anaemia.

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Microorganism Tests, Escherichia coli, Specie: Escherichia coli

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Compact vortex mixer that will accommodate single tubes or a whole handful at once. Two modes, 'touch' or continuous operation, make this ideal for any experiments that require vigorous mixing.

Supplier: EDVOTEK

This experiment introduces chromatographic separation to the class and shows students how dyes of different colours separate on the basis of their size and shape. Columns may be rinsed and reused.

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Ready-to-Load™ kit for teaching DNA fingerprinting and to show how DNA evidence is used in modern forensics. This experiment allows for varied results depending on the selection of DNA fingerprinting patterns.

Supplier: EDVOTEK

This lab activity shows students what real chromosomal DNA looks like and allows them to explore the procedures involved in DNA extraction.

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Basic concepts of DNA fingerprinting are featured in this Ready-to-Load™ kit by comparing crime scene DNA with suspect DNAs. Fingerprint patterns are separated by agarose gel electrophoresis and the students determine who may have commited the crime.

Supplier: EDVOTEK

Kit for teaching students how to extract and precipitate their own DNA. Students can transfer their DNA to a tube that can be used as a pendant on a necklace.